Ordering Recommendation

Assess risk for fetal and neonatal alloimmune thrombocytopenia. May be ordered for parental, fetal, or neonatal genotyping.

Mnemonic

HPA GENO

Methodology

Polymerase Chain Reaction/Fluorescence Monitoring

Performed

Varies

Reported

7-14 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Fetal genotyping: Amniotic fluid
Cultured amniocytes: Two T-25 flasks at 80 percent confluency.
If the client is unable to culture, order test Cytogenetics Grow and Send (ARUP test code 0040182) in addition to this test and ARUP will culture upon receipt (culturing fees will apply). If you have any questions, contact ARUP's Genetics Processing at 800-522-2787 ext. 3301.
WITH maternal cell contamination specimen:
Lavender (EDTA), Pink (K2EDTA), or Yellow (ACD Solution A or B).
Parental genotyping:
Lavender (EDTA).

Specimen Preparation

Amniotic fluid: Transport 10 mL amniotic fluid in a sterile container. (Min: 5 mL)
OR Cultured amniocytes: Transport two T-25 flasks at 80 percent confluency filled with culture media. Backup cultures must be retained at the client's institution until testing is complete.
Maternal cell contamination specimen:
Transport 3 mL whole blood. (Min: 1 mL)
Whole blood (parental genotyping):
Transport 3 mL whole blood. (Min: 1 mL)

Storage/Transport Temperature

Amniotic fluid, cultured amniocytes: CRITICAL ROOM TEMPERATURE. Must be received within 48 hours of shipment due to lability of cells.
Whole blood or maternal cell contamination specimen:
Refrigerated.

Unacceptable Conditions

Frozen specimens in glass collection tubes.

Remarks
Stability

Fetal specimens Amniotic fluid or cultured amniocytes: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Whole blood or maternal cell contamination specimen:
Ambient: 72 hours; Refrigerated: 1 week; Frozen: 1 month

Reference Interval

Interpretive Data

Refer to report

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the U.S. Food and Drug Administration. This test was performed in a CLIA- certified laboratory and is intended for clinical purposes.
Counseling and informed consent are recommended for genetic testing. Consent forms are available online.


PA 1-6, 15 Polymorphism
HPA System
"a" Allele Common
"b" Allele Variant
HPA 1 T C
HPA 2 C T
HPA 3 T G
HPA 4 G A
HPA 5 G A
HPA 6 G A
HPA 15 C A

Compliance Category

Laboratory Developed Test (LDT)

Note

Maternal specimen is recommended for proper test interpretation if contamination of the fetal specimen from the mother is suspected. Order Maternal Cell Contamination.

Hotline History

N/A

CPT Codes

81105; 81106; 81107; 81108; 81109; 81110; 81112

Components

Component Test Code* Component Chart Name LOINC
3001171 Platelet Antigen Geno Interpretation
3001173 Platelet Antigen 1 Genotyping
3001174 Platelet Antigen 2 Genotyping
3001175 Platelet Antigen 3 Genotyping
3001176 Platelet Antigen 4 Genotyping
3001177 Platelet Antigen 5 Genotyping
3001178 Platelet Antigen 6 Genotyping
3001179 Platelet Antigen 15 Genotyping
3001180 Platelet Antigen Geno Specimen
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • HPA platelet antigen genotyping panel
Platelet Antigen Genotyping Panel