Ordering Recommendation
Assess risk for fetal and neonatal alloimmune thrombocytopenia. May be ordered for parental, fetal, or neonatal genotyping.
Mnemonic
Methodology
Polymerase Chain Reaction/Fluorescence Monitoring
Performed
Varies
Reported
7-14 days
New York DOH Approval Status
Specimen Required
Fetal genotyping: Amniotic fluid
Cultured amniocytes: Two T-25 flasks at 80 percent confluency.
If the client is unable to culture, order test Cytogenetics Grow and Send (ARUP test code 0040182) in addition to this test and ARUP will culture upon receipt (culturing fees will apply). If you have any questions, contact ARUP's Genetics Processing at 800-522-2787 ext. 3301.
WITH maternal cell contamination specimen: Lavender (EDTA), Pink (K2EDTA), or Yellow (ACD Solution A or B).
Parental genotyping: Lavender (EDTA).
Amniotic fluid: Transport 10 mL amniotic fluid in a sterile container. (Min: 5 mL)
OR Cultured amniocytes: Transport two T-25 flasks at 80 percent confluency filled with culture media. Backup cultures must be retained at the client's institution until testing is complete.
Maternal cell contamination specimen: Transport 3 mL whole blood. (Min: 1 mL)
Whole blood (parental genotyping): Transport 3 mL whole blood. (Min: 1 mL)
Amniotic fluid, cultured amniocytes: CRITICAL ROOM TEMPERATURE. Must be received within 48 hours of shipment due to lability of cells.
Whole blood or maternal cell contamination specimen: Refrigerated.
Frozen specimens in glass collection tubes.
Fetal specimens Amniotic fluid or cultured amniocytes: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Whole blood or maternal cell contamination specimen: Ambient: 72 hours; Refrigerated: 1 week; Frozen: 1 month
Reference Interval
Interpretive Data
Refer to report
This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the U.S. Food and Drug Administration. This test was performed in a CLIA- certified laboratory and is intended for clinical purposes.
Counseling and informed consent are recommended for genetic testing. Consent forms are available online.
PA 1-6, 15 Polymorphism | ||
---|---|---|
HPA System |
"a" Allele Common |
"b" Allele Variant |
HPA 1 | T | C |
HPA 2 | C | T |
HPA 3 | T | G |
HPA 4 | G | A |
HPA 5 | G | A |
HPA 6 | G | A |
HPA 15 | C | A |
Laboratory Developed Test (LDT)
Note
Maternal specimen is recommended for proper test interpretation if contamination of the fetal specimen from the mother is suspected. Order Maternal Cell Contamination.
Hotline History
Hotline History
CPT Codes
81105; 81106; 81107; 81108; 81109; 81110; 81112
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
3001171 | Platelet Antigen Geno Interpretation | |
3001173 | Platelet Antigen 1 Genotyping | |
3001174 | Platelet Antigen 2 Genotyping | |
3001175 | Platelet Antigen 3 Genotyping | |
3001176 | Platelet Antigen 4 Genotyping | |
3001177 | Platelet Antigen 5 Genotyping | |
3001178 | Platelet Antigen 6 Genotyping | |
3001179 | Platelet Antigen 15 Genotyping | |
3001180 | Platelet Antigen Geno Specimen |
Aliases
- HPA platelet antigen genotyping panel