Ordering Recommendation

For evaluation of optic neuritis, acute myelitis, spinal cord lesions, or autoimmune encephalitis.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum Separator Tube (SST).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Storage/Transport Temperature


Unacceptable Conditions

Amniotic fluid, CSF, pericardial fluid, ocular fluid, peritoneal fluid, synovial fluid, or plasma. Contaminated, hemolyzed, icteric, or lipemic specimens.


After separation from cells: Ambient: 72 hours; Refrigerated: 2 weeks; Frozen: 1 month (avoid repeated freeze/thaw cycles)


Semi-Quantitative Enzyme-Linked Immunosorbent Assay/ Semi-Quantitative Indirect Fluorescent Antibody


Tue, Thu


1-6 days

Reference Interval

Test Number
Reference Interval
  Aquaporin-4 Receptor Antibody 2.9 U/mL or less

Interpretive Data

Approximately 75 percent of patients with neuromyelitis optica (NMO) express antibodies to the aquaporin-4 (AQP4) receptor.  Diagnosis of NMO requires the presence of longitudinally extensive acute myelitis (lesions extending over 3 or more vertebral segments) and optic neuritis.  While absence of antibodies to the AQP4 receptor does not rule out the diagnosis of NMO, presence of this antibody is diagnostic for NMO.

Component Interpretation
Aquaporin-4 Receptor Antibody Negative

2.9 U/mL or less

3.0 U/mL or greater

Compliance Category



If AQP4 antibody IgG by ELISA is positive, then AQP4 antibody IgG by IFA will be added. If AQP4 antibody IgG by IFA is positive, then an AQP4 antibody IgG titer will be added. Additional charges apply.

Hotline History


CPT Codes

86051; if reflexed, add 86052; 86256


Component Test Code* Component Chart Name LOINC
2003121 Aquaporin-4 Receptor Antibody 68548-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


Aquaporin-4 Receptor Antibody by ELISA with Reflex to Aquaporin-4 Receptor Antibody, IgG by IFA