Ordering Recommendation

Use to identify prognostically important abnormalities in newly diagnosed AML. Use to monitor response to therapy with specific probes (CHR FISHI) or progression of disease with probe panel.

Mnemonic
FISH AML
Methodology

Fluorescence in situ Hybridization (FISH)

Performed

Sun-Sat

Reported

3-10 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Non-diluted bone marrow aspirate collected in a heparinized syringe. Also acceptable: Whole blood in green (sodium heparin).
Other specimen types may be acceptable, contact the Cytogenetics Laboratory for specific specimen collection and transportation instructions.

Specimen Preparation

Transfer bone marrow to a green (sodium heparin). Transport 3 mL bone marrow. (Min: 1 mL) OR Transport 5 mL whole blood. (Min: 2 mL)

Storage/Transport Temperature

Room temperature

Unacceptable Conditions

Clotted or paraffin-embedded specimens.

Remarks
Stability

Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable

Reference Interval

Normal

Interpretive Data

Probes included: RPN1/MECOM (EVI1), EGR1, D7S486, RUNX1T1/RUNX1, KMT2A (MLL), and CBFB. The probe for PML/RARα is added automatically and is performed along with the panel. However, due to the longer turnaround time required for the panel, the result of this probe is reported as soon as available and is charged separately. (See PML-RARA Translocation by FISH, ARUP test code 2002363)

Compliance Category

Laboratory Developed Test (LDT)

Note

If cell pellets or dropped cytogenetic slides are submitted, processing fee will not apply.
Other specimen types may be acceptable, contact the Cytogenetics Laboratory for specific specimen collection and transportation instructions.

A processing fee will be charged if this procedure is canceled, at the client's request, after the test has been set up, or if the specimen integrity is inadequate to allow culture growth. The fee will vary based on specimen type.

Hotline History
N/A
CPT Codes

88271 x6; 88275 x6; 88291

Components
Component Test Code* Component Chart Name LOINC
2002385 FISH AML Panel
2011228 EER AML Panel by FISH
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • 'Fluorescence in situ hybridization
  • AML Panel
  • FISH
  • myelogenous leukemia
  • oncology FISH probes
  • t(15;17)
Acute Myeloid Leukemia Panel by FISH