Ordering Recommendation
Use to identify prognostically important abnormalities in newly diagnosed AML. Use to monitor response to therapy with specific probes (CHR FISHI) or progression of disease with probe panel.
Mnemonic
Methodology
Fluorescence in situ Hybridization (FISH)
Performed
Sun-Sat
Reported
3-10 days
New York DOH Approval Status
Specimen Required
Non-diluted bone marrow aspirate collected in a heparinized syringe. Also acceptable: Whole blood in green (sodium heparin).
Other specimen types may be acceptable, contact the Cytogenetics Laboratory for specific specimen collection and transportation instructions.
Transfer bone marrow to a green (sodium heparin). Transport 3 mL bone marrow. (Min: 1 mL) OR Transport 5 mL whole blood. (Min: 2 mL)
Room temperature
Clotted or paraffin-embedded specimens.
Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable
Reference Interval
Normal
Interpretive Data
Probes included: RPN1/MECOM (EVI1), EGR1, D7S486, RUNX1T1/RUNX1, KMT2A (MLL), and CBFB. The probe for PML/RARα is added automatically and is performed along with the panel. However, due to the longer turnaround time required for the panel, the result of this probe is reported as soon as available and is charged separately. (See PML-RARA Translocation by FISH, ARUP test code 2002363)
This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Laboratory Developed Test (LDT)
Note
If cell pellets or dropped cytogenetic slides are submitted, processing fee will not apply.
Other specimen types may be acceptable, contact the Cytogenetics Laboratory for specific specimen collection and transportation instructions.
A processing fee will be charged if this procedure is canceled, at the client's request, after the test has been set up, or if the specimen integrity is inadequate to allow culture growth. The fee will vary based on specimen type.
Hotline History
Hotline History
CPT Codes
88271 x6; 88275 x6
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
2002385 | FISH AML Panel | |
2011228 | EER AML Panel by FISH |
Aliases
- 'Fluorescence in situ hybridization
- AML Panel
- FISH
- myelogenous leukemia
- oncology FISH probes
- t(15;17)