Ordering Recommendation

Use to obtain quantitative results or to follow up a presumptive result. For general screening, the preferred test is Buprenorphine, Urine Screen with Reflex to Quantitation (2012273).

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Random urine.

Specimen Preparation

Transfer 2 mL urine with no additives or preservatives to an ARUP standard transport tube. (Min: 1 mL)

Storage/Transport Temperature

Room temperature.

Unacceptable Conditions

Ambient: 1 week; Refrigerated: 1 month; Frozen: 3 years (Avoid repeated freeze/thaw cycles)


Quantitative Liquid Chromatography-Tandem Mass Spectrometry




1-5 days

Reference Interval

Effective August 17, 2015

Drugs Covered Cutoff Concentrations
Buprenorphine 2 ng/mL
Norbuprenorphine 2 ng/mL
Buprenorphine glucuronide 5 ng/mL
Norbuprenorphine glucuronide 5 ng/mL
Naloxone 100 ng/mL

Interpretive Data

Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry

Positive cutoff:
Buprenorphine: 2 ng/mL
Norbuprenorphine: 2 ng/mL
Buprenorphine glucuronide: 5 ng/mL
Norbuprenorphine glucuronide: 5 ng/mL
Naloxone: 100 ng/mL

For medical purposes only; not valid for forensic use.

The presence of metabolite(s) without parent drug is common and may indicate use of parent drug during the prior week. Naloxone is included to detect the addition of a naloxone-containing drug directly into the urine.

The absence of expected drug(s) and/or drug metabolite(s) may indicate noncompliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, diluted/adulterated urine, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.

Compliance Category

Laboratory Developed Test (LDT)


Hotline History


CPT Codes

80348 (Alt code: G0480)


Component Test Code* Component Chart Name LOINC
2002750 Norbuprenorphine, Urn, Quant 49753-7
2002752 Buprenorphine, Urn, Quant 3415-7
2005863 Naloxone, Urn, Quant 77207-9
2010093 Buprenorphine Gluc, Urn, Quant 3415-7
2010094 Norbuprenorphine Gluc, Urn, Quant 49751-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Belbuca
  • Buprenex
  • Butrans
  • Medication Adherence
  • Narcan
  • Opioids use disorders
  • Pain Management
  • Probuphine
  • Sublocade
  • Suboxone
  • Subutex
  • Temgesic
  • Transtec
  • Vetergesic
  • Zubsolv
Buprenorphine and Metabolites, Urine, Quantitative