Preferred reflex panel for the workup of suspected vasculitis. For patients with a history of vasculitis, refer to Anti-Neutrophil Cytoplasmic Antibody with Reflex to Titer and MPO/PR3 Antibodies (2002068).
For evaluation of autoimmune liver disease, use in conjunction with Autoimmune Liver Disease Evaluation with Reflex to Smooth Muscle Antibody (SMA), IgG by IFA (2007210).
Semi-Quantitative Indirect Fluorescent Antibody/Semi-Quantitative Multiplex Bead Assay
Serum separator tube.
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)
CSF, plasma, urine, or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
|Anti-Neutrophil Cytoplasmic Ab, IgG||< 1:20: Not significant|
|Myeloperox Antibodies, IgG||
|Serine Proteinase 3, IgG||
Neutrophil cytoplasmic antibodies (C-ANCA = granular cytoplasmic staining, P-ANCA = perinuclear staining) are found in the serum of over 90 percent of patients with certain necrotizing systemic vasculitides, and usually in less than 5 percent of patients with collagen vascular disease or arthritis. Approximately 90 percent of patients with a P-ANCA pattern by IFA have antibodies specific for MPO. Approximately 85 percent of patients with a C-ANCA pattern by IFA have antibodies specific for PR3.
No compliance statements are in use for this test.
Specimens are screened for ANCA, MPO and PR3. If the ANCA screen detects antibodies at a 1:20 dilution or greater, then a titer to end point will be added. Additional charges apply.
ANCA IFA is simultaneously tested on ethanol- and formalin-fixed slides to allow differentiation of C- and P-ANCA patterns.
83516 x2; 86255; if reflexed, add 86256
|Component Test Code*||Component Chart Name||LOINC|
|0050526||Myeloperox Antibodies, IgG||6969-0|
|0050527||Serine Proteinase 3, IgG||6968-2|
|0050811||Anti-Neutrophil Cytoplasmic Ab, IgG||29967-7|
- (Perinuclear Antineutrophil Cytoplasmic Antibody
- ANCA Reflex
- Anti-Neutrophil Cytoplasmic Ab Test
- Anti-Neutrophil Cytoplasmic Antibodies Panel, IgG
- Anticytoplasmic Antibody
- Antineutrophil Cytoplasmic Antibodies (ACPA)
- Autoantibodies to Proteinase 3
- Cytoplasmic Neutrophil Antibodies
- Proteinase 3