ANCA-Associated Vasculitis Profile (ANCA/MPO/PR3) with Reflex to ANCA Titer (INACTIVE as of 08/16/21: Refer to 3003745 in the August Hotline)
Preferred reflex panel for the workup of suspected vasculitis. For patients with a history of vasculitis, refer to Anti-Neutrophil Cytoplasmic Antibody with Reflex to Titer and MPO/PR3 Antibodies (2002068).
For evaluation of autoimmune liver disease, use in conjunction with Autoimmune Liver Disease Reflexive Panel (3002479).
Semi-Quantitative Indirect Fluorescent Antibody/Semi-Quantitative Multiplex Bead Assay
New York DOH Approval Status
Serum separator tube.
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)
CSF, plasma, urine, or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
|Anti-Neutrophil Cytoplasmic Ab, IgG||< 1:20: Not significant|
|Myeloperox Antibodies, IgG||
|Serine Proteinase 3, IgG||
Neutrophil cytoplasmic antibodies (C-ANCA = granular cytoplasmic staining, P-ANCA = perinuclear staining) are found in the serum of over 90 percent of patients with certain necrotizing systemic vasculitides, and usually in less than 5 percent of patients with collagen vascular disease or arthritis. Approximately 90 percent of patients with a P-ANCA pattern by IFA have antibodies specific for MPO. Approximately 85 percent of patients with a C-ANCA pattern by IFA have antibodies specific for PR3.
Specimens are screened for ANCA, MPO and PR3. If the ANCA screen detects antibodies at a 1:20 dilution or greater, then a titer to end point will be added. Additional charges apply.
ANCA IFA is simultaneously tested on ethanol- and formalin-fixed slides to allow differentiation of C- and P-ANCA patterns.
83516 x2; 86255; if reflexed, add 86256
|Component Test Code*||Component Chart Name||LOINC|
|0050526||Myeloperox Antibodies, IgG||6969-0|
|0050527||Serine Proteinase 3, IgG||6968-2|
|0050811||Anti-Neutrophil Cytoplasmic Ab, IgG||29967-7|
- (Perinuclear Antineutrophil Cytoplasmic Antibody
- ANCA Reflex
- Anti-Neutrophil Cytoplasmic Ab Test
- Anti-Neutrophil Cytoplasmic Antibodies Panel, IgG
- Anticytoplasmic Antibody
- Antineutrophil Cytoplasmic Antibodies (ACPA)
- Autoantibodies to Proteinase 3
- Cytoplasmic Neutrophil Antibodies
- Proteinase 3