This quantitative test is appropriate for diagnosis and therapeutic monitoring for CML or ALL. The BCR-ABL1 major (p210) fusion forms are present in almost all cases of CML and in a small subset of cases of ALL.
Quantitative Reverse Transcription Polymerase Chain Reaction
RNA isolation: Sun-Sat
Assay: Mon, Wed-Fri
Lavender (EDTA) or bone marrow (EDTA). Also acceptable: RNA extracted by CLIA certified lab.
Whole Blood: Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow: Transport 3 mL bone marrow. (Min: 1 mL)
Specimens must be received within 48 hours of collection due to lability of RNA.
Extracted RNA: Transport 40 uL RNA with at least 40 ng/uL concentration. (Min: 40 uL) Transport RNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Whole Blood or Bone Marrow: CRITICAL REFRIGERATED. Separate specimens must be submitted when multiple tests are ordered.
Extracted RNA: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Serum, plasma, CSF, extracted DNA, RNA extracted by a non-CLIA lab, bone core, or FFPE tissue. Specimens collected in anticoagulants other than EDTA. Severely hemolyzed or clotted specimens.
Ambient: 1 hour; Refrigerated: 48 hours; Frozen: Unacceptable
Extracted RNA: Ambient: Unacceptable; Refrigerate: Unacceptable; Frozen: Indefinitely
Refer to report.
Compliance Statement D: For laboratory tests using a manufactured RUO kit. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
This test does not detect the rare BCR-ABL1 micro (p230) fusion form.
For the p190 fusion form (minor breakpoint), order BCR-ABL1, Minor (p190), Quantitative (ARUP test code 2005016).
|Component Test Code*||Component Chart Name||LOINC|
|0051095||BCR-ABL1, t(9;22) Source||31208-2|
|2005012||BCR-ABL1, Major (p210) Result||42714-6|
|2005014||BCR-ABL1, International Scale (Percent)||69380-4|
|2005015||EER BCR-ABL1, Major (p210)||11526-1|
- CML RT-PCR
- Philadelphia Chromosome Ph1 Bone Marrow/Blood