This quantitative test is appropriate for diagnosis and therapeutic monitoring for CML or ALL. The BCR-ABL1 major (p210) fusion forms are present in almost all cases of CML and in a small subset of cases of ALL.
Quantitative Reverse Transcription Polymerase Chain Reaction
RNA isolation: Sun-Sat
Assay: Mon, Wed-Fri
Lavender (EDTA) or bone marrow (EDTA). Also acceptable: RNA extracted by CLIA certified lab.
Whole Blood: Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow: Transport 3 mL bone marrow. (Min: 1 mL)
Specimens must be received within 48 hours of collection due to lability of RNA.
Extracted RNA: Transport 40 uL RNA with at least 40 ng/uL concentration. (Min: 40 uL) Transport RNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Whole Blood or Bone Marrow: CRITICAL REFRIGERATED. Separate specimens must be submitted when multiple tests are ordered.
Extracted RNA: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Serum, plasma, CSF, extracted DNA, RNA extracted by a non-CLIA lab, bone core, or FFPE tissue. Specimens collected in anticoagulants other than EDTA. Severely hemolyzed or clotted specimens.
Ambient: 1 hour; Refrigerated: 48 hours; Frozen: Unacceptable
Extracted RNA: Ambient: Unacceptable; Refrigerate: Unacceptable; Frozen: Indefinitely
Refer to report.
Laboratory Developed Test (LDT)
This test does not detect the rare BCR-ABL1 micro (p230) fusion form.
For the p190 fusion form (minor breakpoint), order BCR-ABL1, Minor (p190), Quantitative (ARUP test code 2005016).
|Component Test Code*||Component Chart Name||LOINC|
|0051095||BCR-ABL1, t(9;22) Source||31208-2|
|2005012||BCR-ABL1, Major (p210) Result||42714-6|
|2005014||BCR-ABL1, International Scale (Percent)||69380-4|
|2005015||EER BCR-ABL1, Major (p210)||11526-1|
- CML RT-PCR
- Philadelphia Chromosome Ph1 Bone Marrow/Blood