Ordering Recommendation

This quantitative test is appropriate for diagnosis and therapeutic monitoring for CML or ALL. The BCR-ABL1 major (p210) fusion forms are present in almost all cases of CML and in a small subset of cases of ALL.

Mnemonic
BCR MAJ
Methodology

Quantitative Reverse Transcription Polymerase Chain Reaction

Performed

RNA isolation: Sun-Sat
Assay:
Mon, Wed-Fri

Reported

3-6 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Lavender (EDTA) or bone marrow (EDTA). Also acceptable: RNA extracted by CLIA certified lab.

Specimen Preparation

Whole Blood: Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow:
Transport 3 mL bone marrow. (Min: 1 mL)
Specimens must be received within 48 hours of collection due to lability of RNA.
Extracted RNA:
Transport 40 uL RNA with at least 40 ng/uL concentration. (Min: 40 uL) Transport RNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature

Whole Blood or Bone Marrow: CRITICAL REFRIGERATED. Separate specimens must be submitted when multiple tests are ordered.
Extracted RNA: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions

Serum, plasma, CSF, extracted DNA, RNA extracted by a non-CLIA lab, bone core, or FFPE tissue. Specimens collected in anticoagulants other than EDTA. Severely hemolyzed or clotted specimens.

Remarks
Stability

Ambient: 1 hour; Refrigerated: 48 hours; Frozen: Unacceptable
Extracted RNA: Ambient: Unacceptable; Refrigerate: Unacceptable; Frozen: Indefinitely

Reference Interval
Interpretive Data

Refer to report.

Compliance Category

Laboratory Developed Test (LDT)

Note

This test does not detect the rare BCR-ABL1 micro (p230) fusion form.

For the p190 fusion form (minor breakpoint), order BCR-ABL1, Minor (p190), Quantitative (ARUP test code 2005016).

Hotline History
N/A
CPT Codes

81206

Components
Component Test Code* Component Chart Name LOINC
0051095 BCR-ABL1, t(9;22) Source 31208-2
2005012 BCR-ABL1, Major (p210) Result 42714-6
2005014 BCR-ABL1, International Scale (Percent) 69380-4
2005015 EER BCR-ABL1, Major (p210) 11526-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • CML RT-PCR
  • p210
  • Philadelphia Chromosome Ph1 Bone Marrow/Blood
  • t(9;22)
BCR-ABL1, Major (p210), Quantitative