Quantitative Detection of BCR-ABL1, Major Form (p210)
This quantitative test is appropriate for therapeutic monitoring of BCR-ABL1 major (p210) positive chronic myeloid leukemia (CML) or acute lymphoblastic leukemia/lymphoma (ALL). This test is designed to meet the current National Comprehensive Cancer Network (NCCN) guidelines and is recommended for detection of minimal residual disease (MRD).
For patients with uncertain diagnoses or unknown forms of BCR-ABL1 fusion transcripts, consider ordering Diagnostic Qualitative BCR-ABL1 Assay with Reflex to p190 or p210 Quantitative Assays (3005839).
New York DOH Approval Status
Whole blood or bone marrow in lavender (EDTA).
Whole blood: Transport 5 mL whole blood. (Min: 3 mL)
Bone marrow: Transport 3 mL bone marrow. (Min: 1 mL)
Refrigerate immediately. Specimens must be received within 48 hours of collection due to lability of RNA.
Whole blood and bone marrow: CRITICAL REFRIGERATED. Separate specimens must be submitted when multiple tests are ordered.
Serum, plasma, extracted DNA, CSF, FFPE tissue, ambient whole blood, or frozen whole blood or bone marrow.
Specimens collected in anticoagulants other than EDTA. Severely hemolyzed or clotted specimens.
Ambient bone marrow specimens past 7 days will be canceled.
Refrigerated whole blood or bone marrow specimens past 7 days will be canceled.
This quantitative test is recommended for therapeutic monitoring and detection of minimal residual disease for patients with an established diagnosis. For patients with uncertain diagnoses or unknown forms of BCR-ABL1 fusion transcripts, please order Diagnostic Qualitative BCR-ABL1 Assay with Reflex to p190 or p210 Quantitative Assays (ARUP test code 3005839).
Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: Unacceptable
Reverse Transcription Polymerase Chain Reaction
Refer to report.
Laboratory Developed Test (LDT)
This test does not detect the BCR-ABL1 micro (p230) or minor (p190) fusion transcripts. This test does not detect rare BCR-ABL1 major (p210) forms involving beyond ABL1 exon 2.
For the p190 fusion form (minor breakpoint), order BCR-ABL1, Minor (p190), Quantitative (ARUP test code 2005016).
|Component Test Code*||Component Chart Name||LOINC|
|3005854||Quant BCR-ABL1, Major (p210), Source||31208-2|
|3005855||Quant BCR-ABL1, Major (p210), Result||42714-6|
|3005856||Quant BCR-ABL1, Major (p210), IS Percent||69380-4|
|3005858||Quant BCR-ABL1, Major (p210), EER||11526-1|
- Philadelphia chromosome Ph major, bone marrow/blood
- Quantitative RT-PCR, CML or ALL
- t(9;22) major by quantitative RT-PCR