Order in cases of Philadelphia chromosome positive (Ph+) lymphoblastic leukemia to quantify the BCR-ABL1 p190 fusion form. For CML, use BCR-ABL1, Major (p210), Quantitative, (2005017).
Quantitative Reverse Transcription Polymerase Chain Reaction
RNA isolation: Sun-Sat
Assay: Sun, Tue, Thu
Lavender (EDTA) or bone marrow (EDTA). Also acceptable: RNA extracted by CLIA certified lab.
Whole Blood: Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow: Transport 3 mL bone marrow. (Min: 1 mL)
Specimens must be received within 48 hours of collection due to lability of RNA.
Extracted RNA: Transport 40 uL RNA with at least 40 ng/uL concentration. (Min: 40 uL) Transport RNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Whole Blood or Bone Marrow: CRITICAL REFRIGERATED. Separate specimens must be submitted when multiple tests are ordered.
Extracted RNA: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Serum, plasma, CSF, extracted DNA, RNA extracted by a non-CLIA lab, bone core, or FFPE tissue. Specimens collected in anticoagulants other than EDTA. Severely hemolyzed or clotted specimens.
Ambient: 1 hour; Refrigerated: 48 hours; Frozen: Unacceptable
Extracted RNA: Ambient: Unacceptable; Refrigerate: Unacceptable; Frozen: Indefinitely
Refer to report.
Laboratory Developed Test (LDT)
For p210 fusion form (major breakpoint), order BCR-ABL1, Major (p210), Quantitative (ARUP test code 2005017).
|Component Test Code*||Component Chart Name||LOINC|
|0051095||BCR-ABL1, t(9;22) Source||31208-2|
|2005007||BCR-ABL1, Minor (p190) Result||21823-0|
|2005008||BCR-ABL1/ABL1, Minor (p190) Quant Ratio||52132-8|
|2005009||EER BCR-ABL1, Minor (p190)||11526-1|
- philadelphia chromosome
- Philadelphia MS