Ordering Recommendation

If serology is used to assess late-stage pertussis (>4 weeks), the recommended test is Bordetella pertussis Antibodies, IgA and IgG by ELISA with Reflex to Immunoblot (2001774). CDC-recommended first-line tests for pertussis are Bordetella pertussis/parapertussis by PCR (0065080) and/or Bordetella pertussis Culture (0060117).

Mnemonic
BORD BLOTS
Methodology

Qualitative Immunoblot

Performed

Tue, Fri

Reported

1-5 days

New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Specimen Required
Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Heat-inactivated specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year

Reference Interval
Bordetella pertussis Antibodies, IgA, IgG, and IgM by Immunoblot
Effective February 19, 2013
Components Reference Interval
Bordetella pertussis Antibody, IgG by Immunoblot
Bordetella pertussis Ab, IgG by Immunoblot Interp Negative
B. pertussis, IgG Immunoblot PT100 Negative
B. pertussis, IgG Immunoblot PT Negative
B. pertussis, IgG Immunoblot FHA Negative
Bordetella pertussis Antibody, IgA by Immunoblot
Bordetella pertussis Ab, IgA by Immunoblot Interp Negative
B. pertussis, IgA Immunoblot PT Negative
B. pertussis, IgA Immunoblot FHA Negative
Bordetella pertussis Antibody, IgM by Immunoblot
Bordetella pertussis Ab, IgM by Immunoblot Interp Negative
B. pertussis, IgM Immunoblot PT Negative
B. pertussis, IgM Immunoblot FHA Negative

Interpretive Data

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)

Note

This assay tests for the presence of pertussis toxin (PT), pertussis toxin PT 100 (PT-100), and filamentous hemagglutinin antibody (FHA).

Hotline History
N/A
CPT Codes

86615 x3

Components
Component Test Code* Component Chart Name LOINC
2004317 B. pertussis Ab, IgA Immunoblot Interp 29672-3
2004318 B. pertussis, IgA Immunoblot PT 23830-3
2004319 B. pertussis Ab, IgG Immunoblot Interp 29674-9
2004320 B. pertussis, IgG Immunoblot FHA 43881-2
2004321 B. pertussis, IgG Immunoblot PT 69367-1
2004322 B. pertussis, IgG Immunoblot PT100 20992-4
2004323 B. pertussis Ab, IgM Immunoblot Interp 29673-1
2004324 B. pertussis, IgM Immunoblot FHA 43882-0
2004325 B. pertussis, IgM Immunoblot PT 23832-9
2004397 B. pertussis, IgA Immunoblot FHA 43880-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • B. pertussis Abs immunoblot
  • Pertussis Antibody
  • Pertussis IgA,IgG, IgM Abs Immunoblot
Bordetella pertussis Antibodies, IgA, IgG, and IgM by Immunoblot