Ordering Recommendation

CDC-recommended first-line tests for pertussis are Bordetella pertussis/parapertussis by PCR (0065080) and/or Bordetella pertussis Culture (0060117). However, if serology is used to assess late-stage pertussis (>4 weeks), this is the recommended test.

Mnemonic
BORDPAN2
Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot

Performed

Tue, Fri

Reported

1-5 days

New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Specimen Required
Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL) Parallel testing is preferred, and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Contaminated, heat-inactivated, or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval
Test Number
Components
Reference Interval
  B. pertussis Ab, IgA by ELISA
Test Number
Components
Reference Interval
Bordetella pertussis Antibody, IgA by ELISA Effective February 20, 2018
0.9 IV or less: Negative - No significant level of detectable Bordetella pertussis IgA antibody.
1.0-1.1 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.2 IV or greater: Positive - IgA antibody to Bordetella pertussis detected, which may indicate a current or past exposure/immunization to B. pertussis.
2004316 Bordetella pertussis Antibody, IgA by Immunoblot Negative

2001768 Bordetella pertussis Antibody, IgG by ELISA with Reflex to Immunoblot
Test Number
Components
Reference Interval
Bordetella pertussis Antibody IgG by ELISA Effective February 20, 2018
0.94 IV or less: Negative - No significant level of detectable B. pertussis IgG antibody.
0.95-1.04 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.05 IV or greater: Positive - IgG antibody to B. pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis.
2004327 Bordetella pertussis Antibody, IgG by Immunoblot Refer to report

Interpretive Data

Refer to report.

Compliance Category

Laboratory Developed Test (LDT)

Note

If Bordetella pertussis Antibody, IgA by ELISA is 1.2 IV or greater, then Bordetella pertussis IgA Immunoblot testing will be added; if Bordetella pertussis Antibody, IgG by ELISA is 1.05 IV or greater, then Bordetella pertussis IgG Immunoblot testing will be added. Additional charges apply.

Hotline History
N/A
CPT Codes

86615 x2; if reflexed, add 86615 for each Immunoblot

Components
Component Test Code* Component Chart Name LOINC
2001770 B. pertussis Ab, IgA by ELISA 42328-5
2001782 B. pertussis Ab, IgG by ELISA 42330-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • B. pertussis Abs panel reflex
  • Pertussis Antibody
  • Pertussis IgA and IgG Abs reflex to immunoblot
Bordetella pertussis Antibodies, IgA and IgG by ELISA with Reflex to Immunoblot