Ordering Recommendation

May be used to provide evidence of vaccination or past infection; test does not determine immunity to B. pertussis. CDC-recommended first-line tests for pertussis are Bordetella pertussis/parapertussis by PCR (0065080) and/or Bordetella pertussis Culture (0060117). If serology is used to assess late-stage pertussis, the recommended test is Bordetella pertussis Antibodies, IgA and IgG by ELISA with reflex to immunoblot (2001774).

Mnemonic

BORDG BLOT

Methodology

Qualitative Immunoblot

Performed

Tue, Fri, Sun

Reported

1-4 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Heat-inactivated specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year

Reference Interval

Bordetella pertussis Antibody, IgG by Immunoblot
Effective February 19, 2013
Components
Reference Interval
Bordetella pertussis Ab, IgG by Immunoblot Interp Negative
B. pertussis, IgG Immunoblot PT100 Negative
B. pertussis, IgG Immunoblot PT Negative
B. pertussis, IgG Immunoblot FHA Negative

Interpretive Data



Compliance Category

FDA

Note

This assay tests for the presence of pertussis toxin (PT), pertussis toxin PT 100 (PT-100), and filamentous hemagglutinin antibody (FHA).

Hotline History

N/A

CPT Codes

86615

Components

Component Test Code* Component Chart Name LOINC
2004319 B. pertussis Ab, IgG Immunoblot Interp 50398-7
2004320 B. pertussis, IgG Immunoblot FHA 69754-0
2004321 B. pertussis, IgG Immunoblot PT 69755-7
2004322 B. pertussis, IgG Immunoblot PT100 74766-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • B. pertussis IgG immunoblot
  • Pertussis Antibody
  • Pertussis IgG Ab immunoblot
Bordetella pertussis Antibody, IgG by Immunoblot