Ordering Recommendation

Not a stand-alone test. CDC-recommended first-line tests for pertussis are Bordetella pertussis/parapertussis by PCR (0065080) and/or Bordetella pertussis Culture (0060117). If serology is used to assess late-stage pertussis, the recommended test is Bordetella pertussis Antibodies, IgA and IgG by ELISA with Reflex to Immunoblot (2001774).

Mnemonic
BORDA BLOT
Methodology

Qualitative Immunoblot

Performed

Tue, Fri

Reported

1-5 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Contaminated or heat-inactivated specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year

Reference Interval
Bordetella pertussis Antibody, IgA by Immunoblot
Effective February 19, 2013
Components Reference Interval
Bordetella pertussis Ab, IgA by Immunoblot Interp Negative
B. pertussis, IgA Immunoblot PT Negative
B. pertussis, IgA Immunoblot FHA Negative

Interpretive Data



Compliance Category

FDA

Note

This assay tests for the presence of pertussis toxin (PT) and filamentous hemagglutinin antibody (FHA).

Hotline History
N/A
CPT Codes

86615

Components
Component Test Code* Component Chart Name LOINC
2004317 B. pertussis Ab, IgA Immunoblot Interp 29672-3
2004318 B. pertussis, IgA Immunoblot PT 23830-3
2004397 B. pertussis, IgA Immunoblot FHA 43880-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • B. pertussis IgA immunoblot
  • Pertussis Antibody
  • Pertussis IgA Ab Immunoblot
  • Whooping Cough
Bordetella pertussis Antibody, IgA by Immunoblot