Use to detect and quantitate PML-RARA fusion transcripts in patients with acute promyelocytic leukemia. Use to monitor minimal residual disease and assess the risk of disease relapse.
Reverse Transcription Polymerase Chain Reaction
RNA isolation: Sun-Sat
Assay: Sun, Tue, Thu
New York DOH Approval Status
Lavender (EDTA) or bone marrow (EDTA). Also acceptable: RNA extracted by CLIA certified lab.
Whole Blood: Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow: Transport 3 mL bone marrow. (Min: 1 mL)
Specimens must be received within 48 hours of collection due to lability of RNA.
Extracted RNA: Transport 40uL RNA with at least 40 ng/uL concentration (Min: 40uL). Transport RNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Whole Blood and Bone Marrow: CRITICAL REFRIGERATED. Separate specimens must be submitted when multiple tests are ordered.
Extracted RNA: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Serum, plasma, CSF, extracted DNA, RNA extracted by a non-CLIA lab, bone core, or FFPE tissue. Specimens collected in anticoagulants other than EDTA. Severely hemolyzed or clotted specimens.
Ambient: 1 hour; Refrigerated: 48 hours; Frozen: Unacceptable
Extracted RNA: Ambient: Unacceptable; Refrigerate: Unacceptable; Frozen: Indefinitely
Refer to report.
This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Laboratory Developed Test (LDT)
|Component Test Code*||Component Chart Name||LOINC|
|2002874||PML-RARA Translocation Quant||21785-1|
|3003498||PML-RARA Translocation Source||66746-9|
- AMPL' APL
- APL MRD
- APL Post-consolidation therapy testing
- PML-RARA fusion types, A, B, B variant
- PML-RARA long, short, variable types
- PML-RARA S, L, V forms
- PML_RARA fusion types
- Quantitative PML