3018922
print

PML :: RARA Detection by RT-PCR, Quantitative

PMLQNT

Icon

Time Sensitive

Ordering Recommendation

Use to detect and quantitate PML::RARA fusion transcripts in individuals with acute promyelocytic leukemia. Use to monitor minimal residual disease and assess the risk of disease relapse. This test detects fusion types 1, 2, and 3; it does NOT identify which transcript is detected.

New York DOH Approval Status

Testing is not New York state approved. Specimens from New York clients will be sent out to a New York state-approved laboratory.

Specimen Required

Patient Preparation
Collect

Whole blood or bone marrow in lavender (EDTA).

Specimen Preparation

Whole Blood: Transport 5 mL whole blood. (Min: 3 mL)
Bone Marrow: Transport 3 mL bone marrow. (Min: 1 mL)
Refrigerate immediately. Specimens must be received within 48 hours of collection due to lability of RNA.

Storage/Transport Temperature

Whole Blood and Bone Marrow: CRITICAL REFRIGERATED. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions

Serum, plasma, extracted DNA, CSF, FFPE tissue, ambient whole blood, or frozen whole blood or bone marrow. Specimens collected in anticoagulants other than EDTA. Severely hemolyzed or clotted specimens.
Ambient bone marrow specimens received >7 days post collection will be canceled. Refrigerated whole blood or bone marrow specimens received >7 days post collection will be canceled.

Remarks
Stability

Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: Unacceptable

Methodology

Quantitative Reverse Transcription Polymerase Chain Reaction

Performed

Varies

Reported

2-9 days

Reference Interval

By report.

Interpretive Data

Refer to report.

Compliance Category

Laboratory Developed Test (LDT)

Note

Hotline History

N/A

CPT Codes

81315

Are you an ARUP Client? Click here for your pricing.

Components

Component Test Code* Component Chart Name LOINC
3018929 PML::RARA Translocation, Source 66746-9
3018930 PML::RARA Translocation, Result 21551-7
3018931 PML::RARA Translocation, NCN 21785-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • AMPL' APL
  • APL MRD
  • APL Post-consolidation therapy testing
  • PML-RARA fusion types, A, B, B variant
  • PML-RARA long, short, variable types
  • PML-RARA S, L, V forms
  • PML_RARA fusion types
  • Quantitative PML
  • t(15;17)
  • AMPL' APL
  • PML::RARA fusion types, A, B, B variant
  • PML::RARA long, short, variable types
  • PML::RARA S, L, V forms
  • PML::RARA fusion types
PML::RARA Detection by RT-PCR, Quantitative