Ordering Recommendation

Preferred test to follow-up presumptive results. For general screening, Tramadol, Urine Screen with Reflex to Quantitation (2012297) is preferred.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Random urine.

Specimen Preparation

Transfer 2 mL urine with no additives or preservatives an ARUP Standard Transport Tube. (Min: 1 mL)

Storage/Transport Temperature

Room temperature.

Unacceptable Conditions

Specimens exposed to repeated freeze/thaw cycles.


Ambient: 1 week; Refrigerated: 1 month; Frozen: 3 years


Quantitative Liquid Chromatography-Tandem Mass Spectrometry




1-6 days

Reference Interval

Effective May 2018

Drugs Covered Cutoff Concentrations
Tramadol 25 ng/mL
O-desmethyltramadol 25 ng/mL

Interpretive Data

Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry
Positive cutoff:
          Tramadol: 25 ng/mL
            O-desmethyltramadol: 25 ng/mL

For medical purposes only; not valid for forensic use.

The presence of metabolite(s) without parent drug is common and may indicate use of parent drug during the prior week.

The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, diluted/adulterated urine, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


Hotline History


CPT Codes

80373 (Alt code: G0480)


Component Test Code* Component Chart Name LOINC
2002740 O-desmethyltramadol, Urn, Quant 92639-4
2002769 Tramadol, Urn, Quant 17719-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • O-Desmethyltramadol
  • Ryzolt
  • Tramadol, Quantitative, Urine
  • Tramal
  • Ultracet
  • Ultram
  • urine tramadol concentration
  • urine ultram concentration
Tramadol and Metabolite, Urine, Quantitative