Ordering Recommendation

Preferred single screening test for a one time screening of population born between 1945-1965 and individuals at risk for HCV. Positive results require confirmation by molecular testing (eg, Hepatitis C Virus (HCV) by Quantitative NAAT (3000572) or Hepatitis C Virus (HCV) by Quantitative NAAT with Reflex to HCV Genotype by Sequencing (3000576)).


Qualitative Chemiluminescent Immunoassay




Within 24 hours

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Serum Separator Tube (SST). Also acceptable: Lavender (EDTA) or Pink (K2EDTA).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature


Unacceptable Conditions

Specimens containing particulate material. Severely hemolyzed, heat-inactivated, or lipemic specimens. Heparinized plasma.


After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Indefinitely (avoid freeze/thaw cycles)

Reference Interval


Interpretive Data

This assay should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P).

Hepatitis C Antibody by CIA Index
Negative 0.79 IV or less
Equivocal 0.80 to 0.99 IV
Low Positive 1.00 to 10.99 IV
High Positive 11.00 IV or greater
Index Value (IV) = Anti-HCV signal to cutoff (S/C)

No compliance statements are in use for this test.


For both low-positive and high-positive anti-HCV screening results, ARUP recommends collecting a new specimen and testing for Hepatitis C RNA by PCR to rule out active infection.

Hotline History
Component Test Code* Component Chart Name LOINC
0020099 Hepatitis C Antibody by CIA Interp 13955-0
2002404 Hepatitis C Antibody by CIA Index 48159-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Anti-HCV
  • HCV Ab
  • HCV Antibody
  • HCVAB, Serum
  • Hepatitis C Viral Antibody, Serum
  • non-A, non-B Hepatitis
Hepatitis C Virus Antibody by CIA