Ordering Recommendation

May be useful for individuals with vitamin D insufficiency/deficiency who are not responding to supplementation. Not the test of choice for initial evaluation of vitamin D insufficiency; Vitamin D, 25-hydroxy (0080379) is preferred.

Mnemonic
VITD2D3TMS
Methodology

Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry

Performed

Sun-Sat

Reported

1-4 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Plain red or serum separator tube. Also acceptable: Green (sodium heparin), lavender (EDTA), or pink (K2EDTA).

Specimen Preparation

Transfer 0.5 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Room temperature specimens older than 24 hours.

Remarks
Stability

After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months

Reference Interval

Effective May 16, 2011

1-17 years
Deficiency Less than 20 ng/mL
Optimum level Greater than or equal to 20 ng/mL*
*(Wagner CL et al. Pediatrics 2008; 122: 1142-52.)

18 years and older
Deficiency Less than 20 ng/mL
Insufficiency 20-29 ng/mL
Optimum Level 30-80 ng/mL
Possible Toxicity Greater than 150 ng/mL
(Holick MF et al. JCEM 2011; 96:1911-30)

Interpretive Data

Total Concentrations of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3:
Deficiency: Less than 20 ng/mL
Insufficiency: 20-29 ng/mL
Optimal Level: 30-80 ng/mL
Possible Toxicity: Greater than 150 ng/mL

Separate values for Vitamin D2 and D3 are reported in addition to the total.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Note

ARUP is unable to provide reliable results for specimens from infants (less than one year of age), since highly specialized test methodology is required.  ARUP will refer all infant specimens to a laboratory that is able to perform this methodology.
U.S. Patent No. 8,349,613

Hotline History
N/A
CPT Codes

82306

Components
Component Test Code* Component Chart Name LOINC
2002475 25-Hydroxyvitamin D2 and D3 Total 62292-8
2002476 25-Hydroxyvitamin D3 1989-3
2002477 25-Hydroxyvitamin D2 49054-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • 25-Hydroxy D2 (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum)
  • 25-Hydroxy D3 (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum)
  • 25-Hydroxy Vitamin D (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum)
  • 25-Hydroxycholecalciferol (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum)
  • 25-Hydroxyergocalciferol (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum)
  • 25-OH Vitamin D (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum)
  • Calcidiol (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum)
  • D2 (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum)
  • D3 (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum)
  • Fat Malabsorption (Response to Vitamin D2 Supplement) (25-Hydroxyvitamin D2 and D3 by Tandem Mass Sp
  • Soft-25HDN (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum)
  • Total 25 Hydroxyvitamin D (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum)
  • Total 25-Hydroxycholecalciferol (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum)
  • Total Calcidiol (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum)
  • Total Calcifediol (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum)
  • Vitamin D (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum)
  • Vitamin D2 and D3 (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum)
  • Vitamin D3 (25-Hydroxyvitamin D2 and D3 by Tandem Mass Spectrometry, Serum)
25-Hydroxyvitamin D[2] and D[3] by Tandem Mass Spectrometry, Serum