Ordering Recommendation

Preferred test to diagnose vitamin D insufficiency and monitor response to therapy. Testing is recommended only for patients at risk for vitamin D insufficiency.

Mnemonic
VIT D 25
Methodology

Quantitative Chemiluminescent Immunoassay

Performed

Sun-Sat

Reported

Within 24 hours

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Transfer 1 mL serum  to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

EDTA plasma, tissue or urine. Grossly hemolyzed or lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 72 hours; Refrigerated: 1 week; Frozen: 6 months

Reference Interval
0-17 years
Deficiency Less than 20 ng/mL
Optimum level Greater than or equal to 20 ng/mL*
*(Wagner CL et al. Pediatrics 2008; 122: 1142-52.)

18 years and older
Deficiency Less than 20 ng/mL
Insufficiency 20-29 ng/mL
Optimum Level 30-80 ng/mL
Possible Toxicity Greater than 150 ng/mL

Interpretive Data

This assay accurately quantifies the sum of vitamin D3, 25-Hydroxy and vitamin D2, 25-Hydroxy. 

No compliance statements are in use for this test.

Note
Hotline History
N/A
Components
Component Test Code* Component Chart Name LOINC
0080379 Vitamin D 25 OH 1989-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • D, 25-Hydroxy, Vitamin
  • Ergocalciferol
  • Vit D
  • vitamin D
Vitamin D, 25-Hydroxy