Assess donor genotype.
Polymerase Chain Reaction/Fragment Analysis
Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B). OR bone marrow in lavender (EDTA). OR buccal brushes from donor.
Transport 3 mL whole blood (Min: 1 mL) OR 1 mL bone marrow OR 2 buccal brushes in a sterile, dry tube.
Post-transplantation results will be compared to pre-transplant recipient and donor genotypes, therefore, donor and recipient samples must be obtained and genotyped before the transplant event occurs.
Ambient: 1 week; Refrigerated: 1 month; Frozen: 6 months
Background Information for Chimerism, Donor:
Indication: Monitoring for bone marrow transplant patients; correlation with clinical status and consideration of the interval between bone marrow transplantation and testing is necessary for proper interpretation of results.
Methodology: PCR followed by capillary electrophoresis. Specimens are analyzed using 15 autosomal markers (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, vWa, TPOX, D18S51, D5S818, and FGA) and one gender marker (amelogenin).
Kit Used: AmpFLSTR Identifiler® PCR Amplification Kit, Applied Biosystems.
Limit of Detection: 2% of minor cell population. Diagnostic errors can occur due to rare sequence variations.
Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
See CPT codes under (Chimerism, Recipient Pre-Transplant - test code 2002065)
|Component Test Code*||Component Chart Name||LOINC|
|0050759||Chimerism, Donor, Interpretation|
|2001378||Chimerism, Donor, Specimen|
|2002031||Chimerism, Donor, Recipient's Name|
|2002032||Chimerism, Donor, Informative Loci|
- Bone marrow DNA typing
- Chimerism evaluation
- Pre transplant evaluation
- Transplant analysis
- Transplant evaluation