Chimerism, Post-Transplant

2002066
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Additional Technical Information
Ordering Recommendation

Monitor engraftment of donor cells post allogenic stem cell transplantation.

Mnemonic
STR-POST
Methodology

Polymerase Chain Reaction/Fragment Analysis

Performed

Sun-Sat

Reported

5-9 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B). OR bone marrow in lavender (EDTA).

Specimen Preparation

Transport 3 mL whole blood OR 1 mL bone marrow.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions
Remarks

Post-transplantation results will be compared to pre-transplant recipient and donor genotypes, therefore, donor and recipient specimens must be obtained and genotyped before the transplant event occurs.
If cell sorting is required, refer to Chimerism, Post-Transplant, Sorted Cells (ARUP test code 2002064).

Stability

Ambient: 1 week; Refrigerated: 1 month; Frozen: 6 months

Reference Interval

Type Donor - Donor cells only.
Type Recipient - Recipient cells only.
Mixed - Donor and Recipient cells present.  Semi-quantitative results of percentage of donor and recipient cells will be reported.

Interpretive Data

Background Information for Chimerism, Post-Transplant:
Indication: Monitoring for bone marrow transplant patients; correlation with clinical status and consideration of the interval between bone marrow transplantation and testing is necessary for proper interpretation of results.
Methodology: PCR followed by capillary electrophoresis. Specimens are analyzed using 15 autosomal markers (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, vWa, TPOX, D18S51, D5S818, and FGA) and one gender marker (amelogenin).
Kit Used: AmpFLSTR Identifiler® PCR Amplification Kit, Applied Biosystems.
Limit of Detection: 2% of minor cell population.
Limitations: Diagnostic errors can occur due to rare sequence variations.

Compliance Category

Laboratory Developed Test (LDT)

Note
Hotline History
N/A
CPT Codes

81267

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Components
Component Test Code* Component Chart Name LOINC
0050760 Chimerism,Post, Interpretation
2001379 Chimerism,Post, Specimen
2002039 Chimerism,Post, Margin of Error
2002040 Chimerism,Post, Percent Recipient
2002041 Chimerism,Post, Percent Donor
2002042 Chimerism,Post, Informative Loci
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Bone Marrow DNA typing
  • Bone Marrow DNA Typing Transplant Eval., Post-Transplant
  • Bone Marrow Post-Trans Eval
  • Bone Marrow Transplantation
  • Chimerism Evaluation
  • Transplant Analysis
  • Transplant Evaluation
Chimerism, Post-Transplant