Ordering Recommendation

Aids in the detection and subclassification of hyperandrogenism. Most useful in women and children with moderate/severe hirsutism or hirsutism of any degree when it is sudden in onset or rapidly progressive. Hirsutism Evaluation Panel is generally preferred.

Mnemonic
VIRIL PANEL
Methodology

Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry

Performed

Sun-Sat

Reported

1-4 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube or green (sodium or lithium heparin). Collect between 6-10 a.m. Also acceptable: Lavender (EDTA).

Specimen Preparation

Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min 0.5 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions
Remarks
Stability

Ambient: 2 hours; Refrigerated: 1 week; Frozen: 6 months

Reference Interval
Test Number
Components
Reference Interval
2001638 Androstenedione Effective August 19, 2013
Age Female Male
Premature Infants, 26-28 weeks-Day 4 0.92-2.82 ng/mL 0.92-2.82 ng/mL
Premature Infants, 31-35 weeks-Day 4 0.80-4.46 ng/mL 0.80-4.46 ng/mL
Full-term Infants, 1-7 days 0.20-2.90 ng/mL 0.20-2.90 ng/mL
8-30 days 0.18-0.80 ng/mL 0.18-0.80 ng/mL
1-5 months 0.06-0.68 ng/mL 0.06-0.68 ng/mL
6-24 months Less than 0.15 ng/mL 0.03-0.15 ng/mL
2-3 years Less than 0.16 ng/mL Less than 0.11 ng/mL
4-5 years 0.02-0.21 ng/mL 0.02-0.17 ng/mL
6-7 years 0.02-0.28 ng/mL 0.01-0.29 ng/mL
8-9 years 0.04-0.42 ng/mL 0.03-0.30 ng/mL
10-11 years 0.09-1.23 ng/mL 0.07-0.39 ng/mL
12-13 years 0.24-1.73 ng/mL 0.10-0.64 ng/mL
14-15 years 0.39-2.00 ng/mL 0.18-0.94 ng/mL
16-17 years 0.35-2.12 ng/mL 0.30-1.13 ng/mL
18-39 years 0.26-2.14 ng/mL 0.33-1.34 ng/mL
40 years and older 0.13-0.82 ng/mL 0.23-0.89 ng/mL
Pre-menopausal 0.26-2.14 ng/mL Does Not Apply
Postmenopausal 0.13-0.82 ng/mL Does Not Apply
Tanner Stage I 0.05-0.51 ng/mL 0.04-0.32 ng/mL
Tanner Stage II 0.15-1.37 ng/mL 0.08-0.48 ng/mL
Tanner Stage III 0.37-2.24 ng/mL 0.14-0.87 ng/mL
Tanner Stage IV-V 0.35-2.05 ng/mL 0.27-1.07 ng/mL

2001640 Dehydroepiandrosterone, Serum or Plasma Effective August 19, 2013
Age Female Male
Premature Less than 40 ng/mL Less than 40 ng/mL
0-1 day Less than 11 ng/mL Less than 11 ng/mL
2-6 days Less than 8.7 ng/mL Less than 8.7 ng/mL
7 days-1 month Less than 5.8 ng/mL Less than 5.8 ng/mL
1-5 months Less than 2.9 ng/mL Less than 2.9 ng/mL
6-24 months Less than 1.9 9 ng/mL Less than 2.5 ng/mL
2-3 years Less than 0.85 ng/mL Less than 0.63 ng/mL
4-5 years Less than 1.03 ng/mL Less than 0.95 ng/mL
6-7 years Less than 1.79 ng/mL 0.06-1.93 ng/mL
8-9 years 0.14-2.35 ng/mL 0.10-2.08 ng/mL
10-11 years 0.43-3.78 ng/mL 0.32-3.08 ng/mL
12-13 years 0.89-6.21 ng/mL 0.57-4.10 ng/mL
14-15 years 1.22-7.01 ng/mL 0.93-6.04 ng/mL
16-17 years 1.42-9.00 ng/mL 1.17-6.52 ng/mL
18-39 years 1.33-7.78 ng/mL 1.33-7.78 ng/mL
40 years and older 0.63-4.70 ng/mL 0.63-4.70 ng/mL
Postmenopausal 0.60-5.73 ng/mL Does Not Apply
Tanner Stage I 0.14-2.76 ng/mL 0.11-2.37 ng/mL
Tanner Stage II 0.83-4.87 ng/mL 0.37-3.66 ng/mL
Tanner Stage III 1.08-7.56 ng/mL 0.75-5.24 ng/mL
Tanner Stage IV-V 1.24-7.88 ng/mL 1.22-6.73 ng/mL

0081058 Testosterone, Females or Children Effective August 19, 2013
Age
Female
Male
Premature (26-28 weeks) 5-16 ng/dL 59-125 ng/dL
Premature (31-35 weeks) 5-22 ng/dL 37-198 ng/dL
Newborn 20-64 ng/dL 75-400 ng/dL
1-5 months Less than 20 ng/dL 14-363 ng/dL
6-24 months Less than 9 ng/dL Less than 37 ng/dL
2-3 years Less than 20 ng/dL Less than 15 ng/dL
4-5 years Less than 30 ng/dL Less than 19 ng/dL
6-7 years Less than 7 ng/dL Less than 13 ng/dL
8-9 years 1-11ng/dL 2-8 ng/dL
10-11 years 3-32 ng/dL 2-165 ng/dL
12-13 years 6-50 ng/dL 3-619 ng/dL
14-15 years 6-52 ng/dL 31-733 ng/dL
16-17 years 9-58 ng/dL 158-826 ng/dL
18-39 years 9-55 ng/dL 300-1080 ng/dL
40-59 years 9-55 ng/dL 300-890 ng/dL
60 years and older 5-32 ng/dL 300-720 ng/dL
Premenopausal (18 years and older) 9-55 ng/dL Does Not Apply
Postmenopausal 5-32 ng/dL Does Not Apply
Tanner Stage I 2-17 ng/dL 2-15 ng/dL
Tanner Stage II 5-40 ng/dL 3-303 ng/dL
Tanner Stage III 10-63 ng/dL 10-851 ng/dL
Tanner Stage IV-V 11-62 ng/dL 162-847 ng/dL

Interpretive Data

Total testosterone values may not reflect optimal concentrations in all individuals. Free or bioavailable testosterone measurements may provide supportive information.

Compliance Category

Laboratory Developed Test (LDT)

Note
Hotline History
N/A
CPT Codes

82157; 82626; 84403

Components
Component Test Code* Component Chart Name LOINC
0081058 Testosterone, LC-MS/MS 2986-8
2001639 Androstenedione by TMS 1854-9
2001641 Dehydroepiandrosterone by TMS 2193-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Androstenedione (Virilization Panel 1)
  • Dehydroepiandrosterone (Virilization Panel 1)
  • Testosterone (Virilization Panel 1)
Virilization Panel 1