Bordetella pertussis Antibodies, IgG and IgM by ELISA with Reflex to Immunoblot
If serology is used to assess late-stage pertussis, the recommended test is Bordetella pertussis Antibodies, IgA and IgG by ELISA with Reflex to Immunoblot (2001774). CDC-recommended first-line tests for pertussis are Bordetella pertussis/parapertussis by PCR (0065080) and/or Bordetella pertussis Culture (0060117).
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
New York DOH Approval Status
Serum separator tube.
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."
Bacterially contaminated, heat-inactivated, hemolyzed, icteric, lipemic, or turbid specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year
|B. pertussis Ab, IgG by ELISA||1.04 IV or less|
|B. pertussis Ab, IgM by ELISA||1.1 IV or less|
Recommend that treatment decisions be based on the result of the B. pertussis IgM immunoblot test instead of the ELISA test. B. pertussis IgM test by ELISA may produce false-positive results.
This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
|Bordetella pertussis Antibody IgG by ELISA||0.94 IV or less: Negative - No significant level of detectable B. pertussis IgG antibody.
0.95-1.04 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.05 IV or greater: Positive - IgG antibody to B. pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis.
|Bordetella pertussis Antibody IgM by ELISA||0.9 IV or less: Negative - No significant level of detectable B. pertussis IgM antibody.
1.0-1.1 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.2 IV or greater: Positive - IgM antibody to B. pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis
Laboratory Developed Test (LDT)
If Bordetella pertussis Antibody, IgG by ELISA is 1.05 IV or greater, then Bordetella pertussis IgG Immunoblot testing will be added; if Bordetella pertussis Antibody, IgM by ELISA is 1.2 IV or greater, then Bordetella pertussis IgM Immunoblot testing will be added. Additional charges apply.
86615 x2; if reflexed, add 86615 for each Immunoblot
|Component Test Code*||Component Chart Name||LOINC|
|2001782||B. pertussis Ab, IgG by ELISA||42330-1|
|2001783||B. pertussis Ab, IgM by ELISA||42329-3|
- Pertussis Antibody
- Pertussis IgG, IgM Reflex to immunoblot