Bordetella pertussis Antibodies, IgA, IgG, and IgM by ELISA with Reflex to Immunoblot
If serology is used to assess late-stage pertussis (>4 weeks), the recommended test is Bordetella pertussis Antibodies, IgA and IgG by ELISA with Reflex to Immunoblot (2001774). CDC-recommended first-line tests for pertussis are Bordetella pertussis/parapertussis by PCR (0065080) and/or Bordetella pertussis Culture (0060117).
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
Serum separator tube.
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL) Parallel testing is preferred, and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."
Contaminated, heat-inactivated, or severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year
|B. pertussis Ab, IgA by ELISA||
|2001768||Bordetella pertussis Antibody, IgG by ELISA with Reflex to Immunoblot||
|2001769||Bordetella pertussis Antibody, IgM by ELISA with Reflex to Immunoblot||
Recommend that treatment decisions be based on the result of the B. pertussis IgM immunoblot test instead of the ELISA test. B. pertussis IgM test by ELISA may produce false-positive results.
Laboratory Developed Test (LDT)
If Bordetella pertussis Antibody, IgA by ELISA is 1.2 IV or greater, then Bordetella pertussis IgA Immunoblot testing will be added; if Bordetella pertussis Antibody, IgG by ELISA is 1.05 IV or greater, then Bordetella pertussis IgG Immunoblot testing will be added; If Bordetella pertussis Antibody, IgM by ELISA is 1.2 IV or greater, then Bordetella pertussis IgM Immunoblot testing will be added. Additional charges apply.
86615 x3; if reflexed, add 86615 for each Immunoblot
|Component Test Code*||Component Chart Name||LOINC|
|2001770||B. pertussis Ab, IgA by ELISA||42328-5|
|2001782||B. pertussis Ab, IgG by ELISA||42330-1|
|2001783||B. pertussis Ab, IgM by ELISA||42329-3|
- B. pertussis Abs panel reflex
- Pertussis Abs (IgA, IgG, IgM) reflex to immunoblot
- Pertussis Antibody