Ordering Recommendation

Aid in the diagnosis and prognostication of Crohn disease.


Semi-Quantitative Enzyme-Linked Immunosorbent Assay




1-8 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Serum separator tube (SST).

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 0.5 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Storage/Transport Temperature


Unacceptable Conditions

Plasma, other body fluids, grossly icteric, heat-inactivated, grossly hemolyzed, severely lipemic, or contaminated specimens.


After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year
(avoid repeated freeze/thaw cycles)

Reference Interval
Test Number
Reference Interval
  Saccharomyces cerevisiae Ab (gASCA), IgG
0-44 Units Negative
45-50 Units Equivocal
51 Units or greater Positive

  Laminaribioside Carbohydrate Ab ALCA IgG
0-54 Units Negative
55-60 Units Equivocal
61 Units or greater Positive

  Mannobioside Carbohydrate Ab (AMCA), IgG
0-89 Units Negative
90-100 Units Equivocal
101 Units or greater Positive

  Chitobioside Carbohydrate Ab (ACCA), IgA
0-79 Units Negative
80-90 Units Equivocal
91 Units or greater Positive

Interpretive Data

Anti-glycan serologic markers may serve as an aid in the diagnosis of Crohn disease (CD) and an indicator of disease prognosis. Anti-saccharomyces cerevisiae antibody (gASCA) has the highest diagnostic value and anti-chitobioside carbohydrate antibody (ACCA) has the highest association with more-aggressive disease. Both ACCA and gASCA are associated equally with the need for surgery. When found in combination, these markers have the best diagnostic potential. A combination of two or more of the four anti-glycan markers have a significant association with complications and the need for surgery than any single marker alone. A negative result does not rule out CD.

Compliance Category

Analyte Specific Reagent (ASR)

Hotline History
CPT Codes

86671; 83516 x3

Component Test Code* Component Chart Name LOINC
2001609 Saccharomyces cerevisiae Ab (gASCA), IgG 47321-5
2001610 Mannobioside Carbohydrate Ab (AMCA), IgG 53597-1
2001611 Laminaribioside Carbohydrate Ab ALCA IgG 53596-3
2001612 Chitobioside Carbohydrate Ab (ACCA), IgA 53594-8
2013334 Crohn Disease Prognostic Pan Interp. 62364-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Chitobioside Carbohydrate Antibody (ACCA), IgA
  • Glycan Antibodies
  • IBD Prognostic Panel
  • IBDX
  • Inflammatory Bowel Disease Prognostic Panel
  • Laminaribioside Carbohydrate Antibody (ALCA), IgG
  • Saccharomyces cerevisiae Antibody (gASCA), IgG
Crohn Disease Prognostic Panel