Ordering Recommendation

Use to evaluate vitamin B12 deficiency in individuals with macrocytic or unexplained anemia, or unexplained neurologic disease. Preferred test is Vitamin B12 with Reflex to Methylmalonic Acid, Serum (Vitamin B12 Status) (0055662).


Quantitative Liquid Chromatography-Tandem Mass Spectrometry




1-3 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Plain red or serum separator tube. Also acceptable: Green (sodium heparin), green (lithium heparin), lavender (EDTA), or pink (K2EDTA).

Specimen Preparation

Centrifuge and remove serum or plasma from cells within 2 hours of collection. Transfer 1.2 mL serum or plasma to an ARUP Standard Transport Tube and refrigerate or freeze immediately. (Min: 0.6 mL)

Storage/Transport Temperature


Unacceptable Conditions

Room temperature specimens. Grossly hemolyzed or lipemic specimens.


After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: 1 month

Reference Interval

0.00-0.40 µmol/L

Interpretive Data

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Hotline History
CPT Codes


Component Test Code* Component Chart Name LOINC
0099431 MMA Serum/Plasma, Vitamin B12 Status 13964-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Cobalamin Deficiency (Methylmalonic Acid, Serum or Plasma (Vitamin B12 Deficiency))
  • Methylmalonate (Methylmalonic Acid, Serum or Plasma (Vitamin B12 Deficiency))
  • MMA (Methylmalonic Acid, Serum or Plasma (Vitamin B12 Deficiency))
  • Vitamin B12 Deficiency (Methylmalonic Acid, Serum or Plasma (Vitamin B12 Deficiency))
Methylmalonic Acid, Serum or Plasma (Vitamin B12 Status)