Ordering Recommendation

Preferred reflex test for the evaluation of vitamin B12 deficiency in individuals with macrocytic or unexplained anemia, or unexplained neurologic disease.

Mnemonic
B12 MMA
Methodology

Quantitative Chemiluminescent Immunoassay/Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry

Performed

Sun-Sat

Reported

1-3 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Plain red or serum separator tube. Also acceptable: Green (sodium or lithium heparin).

Specimen Preparation

Protect from light during collection, storage and shipment. Centrifuge and remove serum from cells within 2 hours of collection. Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube and refrigerate or freeze immediately. (Min: 1.5 mL)

Storage/Transport Temperature

Frozen.

Unacceptable Conditions

EDTA plasma. Room temperature specimens. Grossly hemolyzed or lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: 1 month

Reference Interval
Available Separately
Components
Reference Interval
Yes (0070150) Vitamin B12 Effective December 2, 2013
180-914 pg/mL
No Methylmalonic Acid, Serum (Vitamin B12 Deficiency) 0.00-0.40 µmol/L

Interpretive Data



Compliance Category

FDA

Note

If Vitamin B12 is less than 300 pg/mL, then Methylmalonic Acid, Serum (Vitamin B12 Status) will be added. Additional charges apply.

Hotline History
N/A
CPT Codes

82607; if reflexed, add 83921

Components
Component Test Code* Component Chart Name LOINC
0055663 Vitamin B12 Status
0055664 Methylmalonic Acid, Serum
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • B12 (Cobalamin)
  • B12 and MMA reflex
  • B12 Assay
  • B12 MMA
  • B12, Serum
  • CblC
  • Cobalamin
  • Methylcobalamin
  • Vit B12 and MMA
Vitamin B12 with Reflex to Methylmalonic Acid, Serum (Vitamin B12 Status)