Ordering Recommendation

May aid in evaluating the prognosis of malignancy in a variety of tumor specimen types. For cell cycle and DNA ploidy studies in products of conception specimens, refer to Products of Conception, Ploidy by Flow Cytometry (2006178).

New York DOH Approval Status

Testing is not New York state approved. Specimens from New York clients will be sent out to a New York state-approved laboratory.

Specimen Required

Patient Preparation

Tumor tissue. Peripheral blood in lavender (EDTA, pink (K2EDTA), or green (sodium or lithium heparin). Bone marrow in green (sodium or lithium heparin).

Specimen Preparation

Tissue: Paraffin -embedded tissue block enriched with tumor OR Peripheral Blood: Transport 5 mL (Min: 1 mL) with Wright's stained slide OR bone marrow: Transport 2 mL (Min: 1 mL) with Wright's stained slide.
New York State Clients: Formalin fixed paraffin embedded tissue)

Storage/Transport Temperature

Tissue (paraffin embedded), peripheral blood with Wright's stained slide, or bone marrow with Wright's stained slide: Refrigerated or room temperature.

Unacceptable Conditions

Products of conception. No tumor tissue remaining on block. Specimens fixed in Bouin's solution (picric acid), mercuric chloride containing fixatives (e.g., B5, Zenker's solution) or ethanol-based fixatives containing ethylene glycol, acetic acid, or zinc chloride. Clotted or hemolyzed whole blood or bone marrow. Decalcified specimens.


Provide the clinical information (pathology report) and specimen source.
If multiple specimens (blocks or slides) are sent to ARUP, they must be accompanied by one of the following: an order comment indicating that the ARUP pathologist should choose the specimen most appropriate for testing (e.g., "Choose best block"), or individual orders for each sample submitted. A Pathologist Block Selection Fee (ARUP test code 3002076) will be added to orders that utilize the first option. If multiple specimens are sent to ARUP without a request for pathologist block/slide selection or individual orders, they will be held until clarification is provided.


Tissue (paraffin embedded): Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable 

Peripheral blood with Wright's stained slide or bone marrow with Wright's stained slide:
Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable


Quantitative Flow Cytometry


Tue, Thu


3-9 days

Reference Interval

Report components include: DNA content, S-phase percent , and copy of histogram.

Interpretive Data

The diagnostic and prognostic importance of tumor DNA content depends on the tumor type and source of tissue.  Interpretative information, if available for the tumor type, is included with the DNA histogram.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the U.S. Food and Drug Administration. This test was performed in a CLIA-certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


This test is suitable for all tumor tissue specimens (including prostate, colon, and breast) except products of conception. For products of conception testing, please refer to DNA Content/Cell Cycle Analysis, Hydatidiform Mole (ARUP test code 2006178).

A thin section of each tissue submitted is stained with H & E to verify the presence of tumor. The DNA content of each tumor is classified as diploid, near-diploid, tetraploid, aneuploid, hypertetraploid, or hypodiploid. The DNA index is the ratio of tumor G0-G1 cells to normal G0-G1 cells.

The tumor-specific S-phase is used when possible. An average histogram S-phase is used for diploid, near-diploid and hypodiploid tumors where the tumor and host S-phases cannot be separated. An average histogram S-phase is also used when the percentage of aneuploid cells in the histogram is low (less than 25 percent).

Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0095737 Source: 31208-2
0095739 DNA Analysis - Ploidy and S-Phase 30911-2
0095814 DNA Analysis S-Phase Percent 41294-0
2008897 DNA Analysis - Index. 30912-0
2010871 EER DNA, Ploidy and S-Phase 11526-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • DNA Ploidy miscellaneous
DNA Cell Cycle Analysis - Ploidy and S-Phase