DNA Cell Cycle Analysis - Ploidy and S-Phase

0095155
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Example Reports
Diploid
Enhanced Report
Near Dip

Ordering Recommendation

May aid in evaluating the prognosis of malignancy in a variety of tumor specimen types. For cell cycle and DNA ploidy studies in products of conception specimens, refer to Products of Conception, Ploidy by Flow Cytometry (2006178).

Mnemonic

DNA MISC

Methodology

Quantitative Flow Cytometry

Performed

Sun, Tue

Reported

3-9 days

New York DOH Approval Status

Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.

Specimen Required

Patient Preparation
Collect

Tumor tissue, body fluid, peripheral blood in Green (Sodium or Lithium Heparin), bone marrow in Green (Sodium or Lithium Heparin), OR urine/bladder washings.

Specimen Preparation

Tissue: Paraffin embed tissue block enriched with tumor
OR Body Fluid: Transport: 100 mL body fluid. (Min: 10 mL)
OR Peripheral Blood: Transport 5 mL whole blood. (Min: 1 mL)
OR Bone Marrow: Transport 2 mL bone marrow. (Min: 1 mL) Specimens with low mononuclear cell counts may require more volume.
OR Urine/Bladder Washings: Centrifuge and remove supernatant. The cell pellet should then be re-suspended in a cell culture media such as Hank's Balanced Salt Solution or RPMI.

Storage/Transport Temperature

Tissue (paraffin embedded), Peripheral Blood or Bone Marrow: Refrigerated
Body Fluid or Urine/Bladder Washings: Refrigerated

Unacceptable Conditions

Products of Conception. No tumor tissue remaining on block. Specimens fixed in Bouin's solution (picric acid), mercuric chloride containing fixatives (e.g., B5, Zenker's solution) or ethanol-based fixatives containing ethylene glycol, acetic acid, or zinc chloride. Clotted or hemolyzed blood or bone marrow. Decalcified specimens.

Remarks

Provide the clinical information (pathology report) and specimen source.
Peripheral Blood, Bone Marrow or Urine/Bladder Washings: Provide a Wright stained slide with specimens.
If multiple specimens (blocks or slides) are sent to ARUP, they must be accompanied by one of the following: an order comment indicating that the ARUP pathologist should choose the specimen most appropriate for testing (e.g., "Choose best block"), or individual orders for each sample submitted. A Pathologist Block Selection Fee (ARUP test code 3002076) will be added to orders that utilize the first option. If multiple specimens are sent to ARUP without a request for pathologist block/slide selection or individual orders, they will be held until clarification is provided.

Stability

Tissue (paraffin embedded): Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Body Fluid or Urine/Bladder Washings: Ambient: Unacceptable; Refrigerated: 24 hours; Frozen: Unacceptable
Peripheral Blood or Bone Marrow: Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable

Reference Interval

Report components include: DNA content, S-phase percent , and copy of histogram.

Interpretive Data

The diagnostic and prognostic importance of tumor DNA content depends on the tumor type and source of tissue.  Interpretive information, if available for the tumor type, is included with the DNA histogram.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the U.S. Food and Drug Administration. This test was performed in a CLIA-certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)

Note

This test is suitable for all tumor tissue specimens (including prostate, colon, and breast) except Products of Conception. For Products of Conception testing, please refer to DNA Content/Cell Cycle Analysis, Hydatidiform Mole (ARUP test code 2006178).

A thin section of each tissue submitted is stained with H & E to verify the presence of tumor. The DNA content of each tumor is classified as diploid, near-diploid, tetraploid, aneuploid, hypertetraploid, or hypodiploid. The DNA index is the ratio of tumor G0-G1 cells to normal G0-G1 cells.

The tumor-specific S-phase is used when possible. An average histogram S-phase is used for diploid, near-diploid and hypodiploid tumors where the tumor and host S-phases cannot be separated. An average histogram S-phase is also used when the percentage of aneuploid cells in the histogram is low (less than 25 percent).

Hotline History

View Hotline History

 

Hotline History

Date of Change
Test Name Change
Methodology
Performance/Reported Schedule
Specimen Requirements
Reference Interval
Interpretive Data
Note
CPT Code
Component Change
Other Interface Change
New Test
Inactive
02/22/2022
X
N/A

CPT Codes

88182

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Components

Component Test Code* Component Chart Name LOINC
0095737 Source: 31208-2
0095739 DNA Analysis - Ploidy and S-Phase 30911-2
0095814 DNA Analysis S-Phase Percent 41294-0
2008897 DNA Analysis - Index. 30912-0
2010871 EER DNA, Ploidy and S-Phase 11526-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • DNA Ploidy miscellaneous
DNA Cell Cycle Analysis - Ploidy and S-Phase