Distinguish between partial hydatiform mole (PHM) and complete hydatiform mole (CHM).
Quantitative Flow Cytometry
Products of conception in paraffin tissue block.
Paraffin embed products of conception in a tissue block
No tissue remaining on the block. Specimens fixed in Bouin's Solution (picric acid), fixatives containing mercuric chloride (e.g. B5, Zenker's solution) or ethanol-based fixatives containing ethylene glycol, acetic acid, or zinc chloride. Decalcified specimens.
Include H&E stained slide and surgical pathology report.
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Flow Cytometry can be used to help identify partial hydatidiform moles. Partial moles are usually triploid while complete moles are diploid or tetraploid. [Clinical Medicine: Pathology, 2008, 1:61-67]. However, most products of conception are diploid by flow cytometry, so a diploid histogram does not suggest a complete hydatidiform mole unless supported clinically and microscopically. Of 35 cases of histologically apparent partial moles, no complications occurred in those that were triploid. However, 20 percent of those that were diploid had complications (persistence, metastasis). [Am J Ob Gyn, 1987, 157: 969-73]
Laboratory Developed Test (LDT)
A thin section of each tissue submitted is stained with H&E. The DNA content is classified as diploid, triploid, tetraploid or aneuploid. The DNA index is the ratio of the DNA content of abnormal cells compared to normal cells.
|Component Test Code*||Component Chart Name||LOINC|
|2006308||POC - DNA Analysis||30911-2|
|2008898||POC - DNA Index||30912-0|
|2010872||EER DNA, POC||11526-1|
- DNA Ploidy for Hydatidiform Moles
- Partial and Complete Hydatidiform Moles
- Partial or Complete Hydatidiform Moles
- Products of Conception