Ordering Recommendation

Preferred test to follow-up presumptive results. For general screening, Fentanyl, Urine Screen with Reflex to Quantitation (2012284) is preferred.


Quantitative Liquid Chromatography-Tandem Mass Spectrometry




1-4 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Random urine.

Specimen Preparation

Transfer 4 mL urine with no additives or preservatives to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature

Room temperature.

Unacceptable Conditions

Specimens exposed to repeated freeze/thaw cycles.


Ambient: 1 month; Refrigerated: 1 month; Frozen: 9 months

Reference Interval
Drugs Covered Cutoff Concentrations
Fentanyl 1.0 ng/mL
Norfentanyl 1.0 ng/mL

Interpretive Data

Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry
Positive cutoff: 1.0 ng/mL

For medical purposes only; not valid for forensic use.

The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, diluted/adulterated urine, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Hotline History
CPT Codes

80354 (Alt code: G0480)

Component Test Code* Component Chart Name LOINC
2005886 Fentanyl, Urn, Quant 3637-6
2005887 Norfentanyl, Urn, Quant 11075-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Actiq
  • Duragesic
  • Durogesic
  • fentanyl and norfentanyl urine concentrations
  • Fentanyl, Quantitative, with medMATCH, Urine
  • Fentora
  • Innovar
  • Instanyl
  • Ionsys
  • Onsolis
  • Sublimaze
Fentanyl and Metabolite, Urine, Quantitative