Recommended for determination of active or passive nicotine use or for differentiation between nicotine replacement and tobacco use. For a screening test to determine active exposure to nicotine and compliance with smoking-cessation programs, Cotinine Screen, Urine (2007081) is preferred.
Quantitative Liquid Chromatography-Tandem Mass Spectrometry
Transfer 4 mL with no additives or preservatives urine to an ARUP Standard Transport Tube. (Min: 1 mL)
Specimens exposed to repeated freeze/thaw cycles.
Ambient: 10 days; Refrigerated: 10 days; Frozen: 8 months
Effective August 17, 2015
|Drugs Covered||Cutoff Concentrations|
|Cotinine (metabolite)||5 ng/mL|
|3-OH-Cotinine (metabolite)||50 ng/mL|
|Nornicotine (metabolite)||2 ng/mL|
|Anabasine (tobacco biomarker)||3 ng/mL|
Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry
Nicotine 2 ng/mL
Cotinine 5 ng/mL
3-OH-Cotinine 50 ng/mL
Nornicotine 2 ng/mL
Anabasine 3 ng/mL
For medical purposes only; not valid for forensic use.
This test is designed to evaluate recent use of nicotine-containing products. Passive and active exposure cannot be discriminated definitively, although a cutoff of 100 ng/mL cotinine is frequently used for surgery qualification purposes. For smoking cessation programs or compliance testing, the absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, diluted/adulterated urine, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Anabasine is included as a biomarker of tobacco use, versus nicotine replacement. Interpretive questions should be directed to the laboratory.
Laboratory Developed Test (LDT)
80323 (Alt code: G0480)
|Component Test Code*||Component Chart Name||LOINC|
|0092357||Nicotine, Urn, Quant||3854-7|
|0092358||Cotinine, Urn, Quant||10366-3|
|0092359||3-OH-Cotinine, Urn, Quant||33916-8|
|0092360||Nornicotine, Urn, Quant||33917-6|
|0092365||Anabasine, Urn, Quant||33915-0|
- Nicotine and Cotinine, LC/MS/MS, Urine
- Pain Management