Ordering Recommendation

Use to obtain quantitative results or to follow up a presumptive result. For general screening, the preferred test is Methadone Urine Screen with Reflex to Quantitation (2012245).

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Random urine.

Specimen Preparation

Transfer 1 mL with no additives or preservatives urine to an ARUP standard transport tube. (Min: 0.5 mL)

Storage/Transport Temperature

Room temperature.

Unacceptable Conditions

Specimens exposed to repeated freeze/thaw cycles.


Ambient: 1 week; Refrigerated: 1 month; Frozen: 3 years


Quantitative Liquid Chromatography-Tandem Mass Spectrometry




1-7 days

Reference Interval

Drugs Covered Cutoff Concentrations
Methadone 100 ng/mL
EDDP 100 ng/mL

Interpretive Data

Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry

Positive cutoff: 100 ng/mL

For medical purposes only; not valid for forensic use.

The absence of expected drug(s) and/or drug metabolite(s) may indicate noncompliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, diluted/adulterated urine, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.

Compliance Category

Laboratory Developed Test (LDT)


Compare to Pain Management, Methadone, Quantitative, with medMATCH, Urine; Pain Management, Methadone, with Confirmation with medMATCH, Urine.

Hotline History


CPT Codes

80358 (Alt code: G0480)


Component Test Code* Component Chart Name LOINC
2005890 Methadone, Urn, Quant 3774-7
2005891 EDDP, Urn, Quant 50542-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Amidone
  • Dolophine
  • EDDP
  • Heptadon
  • Methadone
  • Methadose
  • Pain Management
  • Physeptone
  • Symoron
Methadone and Metabolite, Urine, Quantitative