Ordering Recommendation

Do not use for evaluation of thiamine sufficiency. Refer to Vitamin B1 (Thiamine), Whole Blood (0080388) for nutritional assessment of thiamine.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

Green (sodium or lithium heparin), lavender (EDTA), plasma separator tube, or pink (K2 EDTA).

Specimen Preparation

Separate plasma from cells within one hour of collection. Transfer 1 mL plasma to an ARUP Standard Transport Tube. (Min: 0.2 mL)

Storage/Transport Temperature

Frozen. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions

Hemolyzed specimens or specimens other than heparin or EDTA plasma.

Remarks
Stability

After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen at -20°C: 6 months

Methodology

Quantitative High Performance Liquid Chromatography (HPLC)

Performed

Sun-Sat

Reported

2-4 days

Reference Interval

4-15 nmol/L

Interpretive Data

Thiamine (vitamin B1) is reported. However, thiamine diphosphate (TDP), the biologically active form of thiamine, is not found in measurable concentrations in plasma, and is best determined in whole blood specimens. Plasma thiamine concentration reflects recent intake rather than body stores.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)

Note

Whole blood is the preferred specimen for thiamine assessment. Approximately 80 percent of thiamine present in whole blood is found in red blood cells. Refer to Vitamin B1 (Thiamine), Whole Blood (0080388).

Hotline History

N/A

CPT Codes

84425

Components

Component Test Code* Component Chart Name LOINC
0080389 Vitamin B1, Plasma 20468-5
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Aliases

  • B1 Vitamin
  • Thiamine
  • Vitamin B1 (Thiamine),
  • VITB1
Vitamin B1 (Thiamine), Plasma