Ordering Recommendation

Use for nutritional assessment of vitamin B1 (thiamine).

Mnemonic
VIT B1 WB
Methodology

Quantitative High Performance Liquid Chromatography

Performed

Sun-Sat

Reported

2-4 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Green (Sodium or Lithium Heparin), Lavender (EDTA), or Pink (K2EDTA).

Specimen Preparation

Transfer 3 mL whole blood to an ARUP Standard Transport Tube. (Min: 0.6 mL)

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions

Any specimen other than whole blood. Plasma separator tubes. Glass tubes. Clotted or non-frozen specimens.

Remarks
Stability

Ambient: Unacceptable; Refrigerated: 4 hours; Frozen: 6 months

Reference Interval

70-180 nmol/L

Interpretive Data

This assay measures the concentration of thiamine diphosphate (TDP), the primary active form of vitamin B1. Approximately 90 percent of vitamin B1 present in whole blood is TDP. Thiamine and thiamine monophosphate, which comprise the remaining 10 percent, are not measured.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Note

Whole blood is the preferred specimen for thiamine assessment.  Approximately 80 percent of thiamine present in whole blood is found in red blood cells. 

Hotline History
N/A
CPT Codes

84425

Components
Component Test Code* Component Chart Name LOINC
0080388 Vitamin B1, Whole Blood 74444-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • B1 Vitamin
  • TDP
  • Thiamin Pyrophosphate
  • Thiamine
  • Thiamine Diphosphate
  • TPP
  • VITB1
Vitamin B1 (Thiamine), Whole Blood