Ordering Recommendation

Use for nutritional assessment of vitamin B1 (thiamine).


Quantitative High Performance Liquid Chromatography (HPLC)/Liquid Chromatography-Tandem Mass Spectrometry




2-4 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Green (Sodium or Lithium Heparin), Lavender (EDTA), or Pink (K2EDTA).

Specimen Preparation

Transfer 3 mL whole blood to an ARUP Standard Transport Tube. (Min: 0.6 mL)

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions

Any specimen other than whole blood. Plasma separator tubes. Glass tubes. Clotted or non-frozen specimens.


Ambient: Unacceptable; Refrigerated: 4 hours; Frozen: 6 months

Reference Interval

70-180 nmol/L

Interpretive Data

This assay measures the concentration of thiamine diphosphate (TDP), the primary active form of vitamin B1. Approximately 90 percent of vitamin B1 present in whole blood is TDP. Thiamine and thiamine monophosphate, which comprise the remaining 10 percent, are not measured.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


Whole blood is the preferred specimen for thiamine assessment.  Approximately 80 percent of thiamine present in whole blood is found in red blood cells. 

Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0080388 Vitamin B1, Whole Blood 74444-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • B1 Vitamin
  • TDP
  • Thiamine
  • Thiamine Diphosphate
  • Thiamine Pyrophosphate
  • TPP
  • VITB1
Vitamin B1 (Thiamine), Whole Blood