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Time Sensitive

Ordering Recommendation

Use this immunoassay to evaluate hypogonadism in cisgender males and to monitor testosterone hormone therapies. Not recommended when low testosterone concentrations, such as those found in children and cisgender females, are expected. For these individuals, testing by mass spectrometry is recommended; refer to Testosterone (Adult Females, Children, or Individuals on Testosterone-Suppressing Hormone Therapy) (0081058). Free or bioavailable testosterone measurements may provide supportive information.

Mnemonic

TESTOS

Methodology

Quantitative Electrochemiluminescent Immunoassay

Performed

Sun-Sat

Reported

Within 24 hours

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Collect specimen between 6-10 a.m.

Collect

Serum Separator Tube (SST) or Green (Lithium Heparin). Also acceptable: Lavender (K2 EDTA) or Lavender (K3 EDTA).
Pooled Specimens: Collect three samples, 20 minutes apart.

Specimen Preparation

Transport 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)
Pooled Specimens: Spin and pool equal amounts of serum or plasma. Transfer 1 mL of pooled specimen into an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature

Refrigerated. Also acceptable: Frozen.

Unacceptable Conditions
Remarks
Stability

After separation from cells: Ambient: 5 days; Refrigerated: 2 weeks; Frozen: 6 months

Reference Interval

Male:
14-15 years: 33-585 ng/dL
16-17 years: 185-886 ng/dL
18-39 years: 300-1080 ng/dL
40-59 years: 300-890 ng/dL
60 years and older: 300-720 ng/dL

Tanner Stage IV: 165-854 ng/dL
Tanner Stage V: 194-783 ng/dL

Interpretive Data

Testosterone immunoassays are both imprecise and inaccurate at low testosterone concentrations, such as those found in children and cisgender females. For these individuals, testing by mass spectrometry is recommended; refer to Testosterone (Adult Females, Children, or Individuals on Testosterone-Suppressing Hormone Therapy) (ARUP test code 0081058). Free or bioavailable testosterone measurements may provide supportive information.

For individuals on testosterone hormone therapy, refer to cisgender male reference intervals. No reference intervals have been established for males younger than 14 years or for cisgender females. For a complete set of all established reference intervals, refer to ltd.aruplab.com/Tests/Pub/0070130.

Compliance Category

FDA

Note

Hotline History

N/A

CPT Codes

84403

Components

Component Test Code* Component Chart Name LOINC
0070130 Testosterone by Immunoassay 2986-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • male testosterone
  • serum total testosterone
  • total testosterone serum
Testosterone (Adult Males or Individuals on Testosterone Hormone Therapy)