Ordering Recommendation

Use in conjunction with free testosterone in the evaluation of suspected hyperandrogenemia in women and children.




Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry




1-4 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Collect between 6-10 a.m.


Serum separator tube or green (sodium or lithium heparin).

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.2 mL)

Storage/Transport Temperature


Unacceptable Conditions

EDTA plasma.


This test is suggested for women and children due to an improved sensitivity of testosterone by LC-MS/MS.


After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months

Reference Interval

Effective August 19, 2013

Premature (26-28 weeks) 5-16 ng/dL 59-125 ng/dL
Premature (31-35 weeks) 5-22 ng/dL 37-198 ng/dL
Newborn 20-64 ng/dL 75-400 ng/dL
1-5 months Less than 20 ng/dL 14-363 ng/dL
6-24 months Less than 9 ng/dL Less than 37 ng/dL
2-3 years Less than 20 ng/dL Less than 15 ng/dL
4-5 years Less than 30 ng/dL Less than 19 ng/dL
6-7 years Less than 7 ng/dL Less than 13 ng/dL
8-9 years 1-11ng/dL 2-8 ng/dL
10-11 years 3-32 ng/dL 2-165 ng/dL
12-13 years 6-50 ng/dL 3-619 ng/dL
14-15 years 6-52 ng/dL 31-733 ng/dL
16-17 years 9-58 ng/dL 158-826 ng/dL
18-39 years 9-55 ng/dL 300-1080 ng/dL
40-59 years 9-55 ng/dL 300-890 ng/dL
60 years and older 5-32 ng/dL 300-720 ng/dL
Premenopausal (18 years and older) 9-55 ng/dL Does Not Apply
Postmenopausal 5-32 ng/dL Does Not Apply
Tanner Stage I 2-17 ng/dL 2-15 ng/dL
Tanner Stage II 5-40 ng/dL 3-303 ng/dL
Tanner Stage III 10-63 ng/dL 10-851 ng/dL
Tanner Stage IV-V 11-62 ng/dL 162-847 ng/dL

Interpretive Data

Total testosterone values may not reflect optimal concentrations in all individuals. Free or bioavailable testosterone measurements may provide supportive information.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0081058 Testosterone, LC-MS/MS 2986-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Total Testosterone
Testosterone, Females or Children