Ordering Recommendation

Obsolete test for prostate cancer screening; preferred test is Prostate Specific Antigen, Total (0070121) in conjunction with digital rectal exam.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Specimen should be obtained before rectal examination, biopsy, prostatectomy or prostatic massage, since manipulation of the prostate gland may lead to elevated PAP levels persisting up to 24-48 hours.


Serum separator tube.

Specimen Preparation

Allow specimen to clot completely at room temperature. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature

Frozen. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions

Specimens refrigerated more than 24 hours or left at room temperature more than 3 hours.


After separation from cells: Ambient: 3 hours; Refrigerated: 24 hours; Frozen: 6 months


Quantitative Chemiluminescent Immunoassay




Within 24 hours

Reference Interval

0.0-3.5 ng/mL

Interpretive Data

The Siemens Immulite 2000 PAP chemiluminescent immunoassay method is used. Results obtained with different assay methods or kits cannot be used interchangeably. PAP may be of some use in predicting disease recurrence or monitoring treatment effects. It has little clinical utility as a screening test for prostate cancer. Prostate specific antigen (PSA) is the preferred test for prostate cancer screening, monitoring, and predicting outcomes. Benign prostatic hyperplasia, prostate massage, and prostatic infarction may result in elevated PAP concentrations. The PAP assay value, regardless of level, should not be interpreted as absolute evidence for the presence or absence of malignant disease.

Compliance Category



Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0070120 Prostatic Acid Phosphatase 20420-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Acid Phosphatase
  • Acid Phosphatase, Prostatic Isoenzyme,
  • PAP
Prostatic Acid Phosphatase