Ordering Recommendation

May be helpful in evaluating for autoimmune liver disease. For a more comprehensive reflex panel, refer to Autoimmune Liver Disease Reflexive Panel (3002479).

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum Separator Tube (SST).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube.(Min: 0.25 mL)

Storage/Transport Temperature


Unacceptable Conditions

Plasma. Contaminated, heat-inactivated, hemolyzed, or lipemic specimens.


After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year


Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody




1-5 days

Reference Interval

Test Number
Reference Interval
  F-Actin (Smooth Muscle) Ab, IgG by ELISA 19 Units or less

Interpretive Data

F-Actin (Smooth Muscle) Antibody, IgG Interpretive Data
19 Units or less Negative
20-30 Units Weak Positive - Suggest repeat testing in two or three weeks with a fresh specimen.
31 Units or greater Positive - Suggestive of autoimmune hepatitis or chronic active hepatitis.

Compliance Category



In viral hepatitis the titers are generally less than 1:80 and are transient. The titers in primary biliary cirrhosis are also low, ranging from 1:20 to 1:40.

If F-Actin is 20 Units or greater, then Smooth Muscle Ab, IgG IFA titer will be added. Additional charges apply

Hotline History


CPT Codes

86015; if reflexed, add 86256


Component Test Code* Component Chart Name LOINC
0055248 F-Actin (Smooth Muscle) Ab, IgG by ELISA 44706-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Actin Antibody
  • Actin Antibody Reflexive Panel
  • Anti-Smooth Muscle Antibody
  • ASMA
  • ASMA Reflexive Panel
  • F-Actin Antibody
  • F-Actin Antibody Panel
  • SMA
  • SMA IgG
  • SMA IgG Ab
  • SMA Reflexive Panel
  • Smooth Muscle Antibodies
  • Soft-SMA
F-Actin (Smooth Muscle) Antibody, IgG by ELISA with Reflex to Smooth Muscle Antibody, IgG Titer