Ordering Recommendation

May be helpful in evaluating for autoimmune liver disease. For a more comprehensive reflex panel, refer to Autoimmune Liver Disease Reflexive Panel (3002479).


Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody




1-5 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Serum Separator Tube (SST).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube.(Min: 0.25 mL)

Storage/Transport Temperature


Unacceptable Conditions

Plasma. Contaminated, heat-inactivated, hemolyzed, or lipemic specimens.


After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year

Reference Interval

Test Number
Reference Interval
  F-Actin (Smooth Muscle) Ab, IgG by ELISA 19 Units or less

Interpretive Data

F-Actin (Smooth Muscle) Antibody, IgG Interpretive Data
19 Units or less Negative
20-30 Units Weak Positive - Suggest repeat testing in two or three weeks with a fresh specimen.
31 Units or greater Positive - Suggestive of autoimmune hepatitis or chronic active hepatitis.

Compliance Category



In viral hepatitis the titers are generally less than 1:80 and are transient. The titers in primary biliary cirrhosis are also low, ranging from 1:20 to 1:40.

If F-Actin is 20 Units or greater, then Smooth Muscle Ab, IgG IFA titer will be added. Additional charges apply

Hotline History


CPT Codes

86015; if reflexed, add 86256


Component Test Code* Component Chart Name LOINC
0055248 F-Actin (Smooth Muscle) Ab, IgG by ELISA 44706-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Actin Antibody
  • Actin Antibody Reflexive Panel
  • Anti-Smooth Muscle Antibody
  • ASMA
  • ASMA Reflexive Panel
  • F-Actin Antibody
  • F-Actin Antibody Panel
  • SMA
  • SMA IgG
  • SMA IgG Ab
  • SMA Reflexive Panel
  • Smooth Muscle Antibodies
  • Soft-SMA
F-Actin (Smooth Muscle) Antibody, IgG by ELISA with Reflex to Smooth Muscle Antibody, IgG Titer