Ordering Recommendation

First-line screening test for ANCA-associated vasculitis. Recommended in the differential evaluation of autoimmune diseases with renal involvement (SLE and anti-glomerular basement membrane disease) and as adjunct diagnostic tool to differentiate ulcerative colitis from Crohn disease in IBD.
A reflex panel is available for the workup of suspected vasculitis; refer to ANCA-Associated Vasculitis Profile (ANCA/MPO/PR3) Reflex to ANCA Titer (2006480).

Mnemonic
ANCA IFA
Methodology

Semi-Quantitative Indirect Fluorescent Antibody

Performed

Sun-Sat

Reported

1-3 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma, urine, or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval

Less than 1:20: Not significant

Interpretive Data

Neutrophil Cytoplasmic Antibodies (C-ANCA = granular cytoplasmic staining, P-ANCA = perinuclear staining) are found in the serum of over 90% of patients with certain necrotizing systemic vasculitides, and usually in less than 5% of patients with collagen vascular disease or arthritis.

No compliance statements are in use for this test.

Note

If the ANCA screen detects antibodies at a 1:20 dilution or greater, then a titer to end point will be added. Additional charges apply. ANCA IFA is simultaneously tested on ethanol- and formalin-fixed slides to allow differentiation of C- and P-ANCA patterns.

Hotline History
N/A
CPT Codes

86255; if positive, add 86256

Components
Component Test Code* Component Chart Name LOINC
0050811 Anti-Neutrophil Cytoplasmic Ab, IgG 29967-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • ANCA
  • ANCA IgG
  • ANCA IgG Ab
Anti-Neutrophil Cytoplasmic Antibody, IgG