First-line screening test for ANCA-associated vasculitis. Recommended in the differential evaluation of autoimmune diseases with renal involvement (SLE and anti-glomerular basement membrane disease) and as adjunct diagnostic tool to differentiate ulcerative colitis from Crohn disease in IBD.
A reflex panel is available for the workup of suspected vasculitis; refer to ANCA-Associated Vasculitis Profile (ANCA/MPO/PR3) Reflex to ANCA Titer (2006480).
Semi-Quantitative Indirect Fluorescent Antibody
Serum separator tube.
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)
Plasma, urine, or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Less than 1:20: Not significant
Neutrophil Cytoplasmic Antibodies (C-ANCA = granular cytoplasmic staining, P-ANCA = perinuclear staining) are found in the serum of over 90% of patients with certain necrotizing systemic vasculitides, and usually in less than 5% of patients with collagen vascular disease or arthritis.
If the ANCA screen detects antibodies at a 1:20 dilution or greater, then a titer to end point will be added. Additional charges apply. ANCA IFA is simultaneously tested on ethanol- and formalin-fixed slides to allow differentiation of C- and P-ANCA patterns.
86255; if positive, add 86256
|Component Test Code*||Component Chart Name||LOINC|
|0050811||Anti-Neutrophil Cytoplasmic Ab, IgG||29967-7|
- ANCA IgG
- ANCA IgG Ab