Detect circulating immune complexes.
Quantitative Flow Cytometry/Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Plain red or serum separator tube (SST).
Allow complete clotting of red blood cells (up to 1 hour), then separate serum from cells within 30 minutes and freeze immediately. Transfer TWO (2) 1 mL aliquots of serum to individual ARUP Standard Transport Tubes. (Min: 0.5 mL/aliquot)
CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Non-frozen specimens. Specimens exposed to repeated freeze/thaw cycles.
Raji: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 30 days
C1q: (After separation from cells): Ambient: 2 hours; Refrigerated: Unacceptable; Frozen: 2 weeks (avoid repeated freeze/thaw cycles)
Less than or equal to 3.9 µg Eq/mL is considered negative for circulating complement binding immune complexes. Circulating immune complexes may be found without any evident pathology and positive results do not necessarily implicate the immune complex in a disease process. Many autoimmune disorders, chronic infections and malignancies are associated with circulating immune complexes. Quantitation of immune complexes assists in staging immunologic disorders.
Laboratory Developed Test (LDT)
Immune complexes bind to Raji Cells via their complement receptors. The immunoglobulin portion of the immune complex is then detected by flow cytometry with anti-human IgG-FITC.
|Component Test Code*||Component Chart Name||LOINC|
|0050301||C1q Binding Assay||27831-7|
|0050302||Raji Cell Immune Complex Assay||10864-7|
- C1q Immune Complexes
- C3d and C1q binding
- Circulating Immune Complex Panel