Ordering Recommendation

Aids in the diagnosis of histoplasmosis. Not recommended as a standalone test. For more complete serologic testing, immunodiffusion should be performed in parallel with complement fixation; refer to Histoplasma Antibodies by Complement Fixation and Immunodiffusion (0050627).

Mnemonic

HISTO

Methodology

Semi-Quantitative Complement Fixation

Performed

Sun-Sat

Reported

2-5 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.4 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of acute specimens. Mark specimens plainly as "acute" or "convalescent."

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Contaminated, hemolyzed, or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval

Test Number
Components
Reference Interval
  Histoplasma Mycelia Antibodies by CF Less than 1:8
  Histoplasma Yeast Antibodies by CF Less than 1:8

Interpretive Data

A titer of 1:8 or greater is generally considered presumptive evidence of histoplasmosis. A titer of 1:32 or greater or rising titers indicate strong presumptive evidence of histoplasmosis. Cross-reactions, usually at lower titers, may occur with other fungal disease.

Compliance Category

FDA

Note

This complement fixation test detects total antibodies to mycelial and yeast antigens of Histoplasma.

Hotline History

N/A

CPT Codes

86698 x2

Components

Component Test Code* Component Chart Name LOINC
0050330 Histoplasma Mycelia Antibodies by CF 20573-2
0050335 Histoplasma Yeast Antibodies by CF 20574-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Histoplasma capsulatum
  • Histoplasmosis
  • Precipitin
Histoplasma Antibodies by Complement Fixation