Ordering Recommendation

Aids in the diagnosis of histoplasmosis. Not recommended as a standalone test. For more complete serologic testing, immunodiffusion should be performed in parallel with complement fixation; refer to Histoplasma Antibodies by Complement Fixation and Immunodiffusion (0050627).

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.4 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of acute specimens. Mark specimens plainly as "acute" or "convalescent."

Storage/Transport Temperature


Unacceptable Conditions

Contaminated, hemolyzed, or severely lipemic specimens.


After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)


Semi-Quantitative Complement Fixation




2-5 days

Reference Interval

Test Number
Reference Interval
  Histoplasma Mycelia Antibodies by CF Less than 1:8
  Histoplasma Yeast Antibodies by CF Less than 1:8

Interpretive Data

A titer of 1:8 or greater is generally considered presumptive evidence of histoplasmosis. A titer of 1:32 or greater or rising titers indicate strong presumptive evidence of histoplasmosis. Cross-reactions, usually at lower titers, may occur with other fungal diseases.

Compliance Category



This complement fixation test detects total antibodies to mycelial and yeast antigens of Histoplasma.

Hotline History


CPT Codes

86698 x2


Component Test Code* Component Chart Name LOINC
0050330 Histoplasma Mycelia Antibodies by CF 20573-2
0050335 Histoplasma Yeast Antibodies by CF 20574-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Histoplasma capsulatum
  • Histoplasmosis
  • Precipitin
Histoplasma Antibodies by Complement Fixation