Aids in the diagnosis of histoplasmosis. Not recommended as a standalone test. For more complete serologic testing, immunodiffusion should be performed in parallel with complement fixation; refer to Histoplasma Antibodies by Complement Fixation and Immunodiffusion (0050627).
Semi-Quantitative Complement Fixation
New York DOH Approval Status
Serum separator tube.
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.4 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of acute specimens. Mark specimens plainly as "acute" or "convalescent."
Contaminated, hemolyzed, or severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
|Histoplasma Mycelia Antibodies by CF||Less than 1:8|
|Histoplasma Yeast Antibodies by CF||Less than 1:8|
A titer of 1:8 or greater is generally considered presumptive evidence of histoplasmosis. A titer of 1:32 or greater or rising titers indicate strong presumptive evidence of histoplasmosis. Cross-reactions, usually at lower titers, may occur with other fungal diseases.
This complement fixation test detects total antibodies to mycelial and yeast antigens of Histoplasma.
|Component Test Code*||Component Chart Name||LOINC|
|0050330||Histoplasma Mycelia Antibodies by CF||20573-2|
|0050335||Histoplasma Yeast Antibodies by CF||20574-0|
- Histoplasma capsulatum