Hemoglobin Evaluation with Reflex to Electrophoresis and/or RBC Solubility
Ordering Recommendation
Effective test for screening and follow up of individuals with known hemoglobinopathies.
Methodology
High Performance Liquid Chromatography (HPLC) /Electrophoresis/RBC Solubility
Performed
Sun-Sat
Reported
1-5 days
New York DOH Approval Status
Specimen Required
Lavender (EDTA) or pink (K2EDTA).
Transport 5 mL whole blood. (Min: 0.2 mL)
Refrigerated.
Frozen or room temperature specimens.
Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Unacceptable
Reference Interval
Effective August 19, 2013
Age-Defined Normal Hemoglobin Reference Intervals |
|||||||
---|---|---|---|---|---|---|---|
Age |
Hb A Percent |
Hb A2 Percent |
Hb F Percent |
Hb S Percent |
Hb C Percent |
Hb E Percent |
Hb Other Percent |
0-1 month 2 months 3 months 4 months 5 months 6-8 months 9-12 months 13-23 months 2 years and older |
7.6-54.8 14.7-70.1 26.6-81.8 43.0-89.5 60.8-94.0 78.2-96.6 86.1-97.2 85.1-97.7 95.0-97.9 |
0.0-1.4 0.0-2.0 0.1-2.6 0.8-3.0 1.5-3.3 1.8-3.5 1.9-3.5 1.9-3.5 2.0-3.5 |
45.8-91.7 32.7-85.2 14.5-73.7 4.2-56.9 1.0-38.1 0.9-19.4 0.6-11.6 0.0-8.5 0.0-2.1 |
0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 |
0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 |
0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 |
0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 |
Interpretive Data
FDA
Note
If abnormal peaks suggestive of a hemoglobin variant are detected, then RBC Solubility and/or Capillary
Electrophoresis will be added to aid in confirmation and identification of the variant. Additional charges apply.
Quantitation of hemoglobin is recommended for a definitive diagnosis in infants 1 year and older.
Additional testing of detected hemoglobin variants may increase turnaround time up to 10 days.
Hotline History
CPT Codes
83021; if reflexed, add 83020; 85660
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
0050295 | Hemoglobin A | 4547-6 |
0050300 | Hemoglobin A2 | 4551-8 |
0050305 | Hemoglobin C | 4563-3 |
0050310 | Hemoglobin E | 4575-7 |
0050315 | Hemoglobin F | 4576-5 |
0050320 | Hemoglobin - Other | 48343-8 |
0050325 | Hemoglobin S | 4625-0 |
0050611 | Hemoglobin Evaluation | 21026-0 |
0081270 | Sickle Cell Solubility | 6864-3 |
2008793 | Hemoglobin, Capillary Electrophoresis | 13514-5 |
Aliases
- Evaluation, Hemoglobin
- Fetal Hemoglobin (Hemoglobin F)
- Hb
- Hb ELP
- Hb IEF
- Hemoglobin Evaluation
- Hemoglobin Fractionation
- Hemoglobinopathy Fractionation Profile
- Hgb
- Sickle Cell Anemia Screen
- Sickle Cell Disease
- Sickle Cell Screen