Ordering Recommendation

Detect circulating immune complexes. Consider ordering Immune Complex Panel (0050667), which contains both Raji cell immune complex and C1q binding assays.

Mnemonic
RAJI
Methodology

Quantitative Flow Cytometry

Performed

Tue

Reported

2-9 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Plain red or serum separator tube (SST).

Specimen Preparation

Allow complete clotting of red blood cells (up to 1 hour), then separate serum from cells within 30 minutes and freeze immediately. Transport 1 mL serum. (Min: 0.5 mL) If ordered in conjunction with a C1q Binding Assay, transfer TWO (2) 1 mL aliquots of serum to individual ARUP Standard Transport Tubes.

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions

Non-frozen specimens. Specimens exposed to repeated freeze/thaw cycles.

Remarks
Stability

Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 30 days

Reference Interval

By report

Interpretive Data

Many autoimmune disorders, chronic infections and malignancies are associated with circulating immune complexes.  Quantitation of immune complexes assists in staging immunologic disorders.

Compliance Category

Laboratory Developed Test (LDT)

Note

Immune complexes bind to Raji Cells via their complement receptors. The immunoglobulin portion of the immune complex is then detected by flow cytometry with anti-human IgG-FITC.

Hotline History
N/A
CPT Codes

86332

Components
Component Test Code* Component Chart Name LOINC
0050302 Raji Cell Immune Complex Assay 10864-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Immune Complex Assay
Raji Cell Immune Complex Assay