Ordering Recommendation

Aids in diagnosis of CSF leak.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Aural or nasal fluid in sterile container. If there is enough secretion, the specimen is collected in a test tube. A specimen can also be obtained by specifically applied suction. Straining, increase in abdominal pressure, or performance of the Valsalva maneuver may result in an increase in drainage. Specimen kept refrigerated may be collected if there is slow or intermittent flow or fluid.

Specimen Preparation

Transport 2 mL aural or nasal fluid in a tube without preservative. (Min: 1 mL aural or nasal fluid)

Storage/Transport Temperature


Unacceptable Conditions

Ambient: 2 weeks; Refrigerated: 2 weeks; Frozen: 2 weeks


Qualitative Immunofixation Electrophoresis




1-4 days

Reference Interval

None detected

Interpretive Data

Detection of a beta-2 transferrin band by IFE is diagnostic for the presence of cerebrospinal fluid (CSF). This test is useful in the differential diagnosis for CSF otorrhea or CSF rhinorrhea. Beta-2 transferrin is not detected by this methodology in normal serum, tears, saliva, sputum, nasal, or aural fluid.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


The beta-2 transferrin protein assay by IFE methodology is not a reliable method for detecting human perilymph due to the low sensitivity of the assay.

Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0050047 Beta-2 Transferrin 13876-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Cerebrospinal Fluid Leakage
  • Transferrin, Spinal Fluid
  • B2 Transferrin
  • CSF Leakage Detection
  • CSF Specific Transferrin
  • CSF-Specific Transferrin
  • Desialated Transferrin
  • Tau CSF Protein
  • Transferrin B2
Beta-2 Transferrin