Ordering Recommendation

May be useful in detecting glomerular basement membrane (GBM) antibodies. Combined use of IFA Glomerular Basement Membrane Antibody, IgG by Multiplex Bead Assay and IFA (2008403) may improve diagnostic sensitivity for disease. If positive, may be useful for monitoring treatment response.

Mnemonic
GBM-G IFA
Methodology

Indirect Fluorescent Antibody

Performed

Sun-Sat

Reported

1-3 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transport 1 mL serum. (Min: 0.5 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions
Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval

Negative

Interpretive Data

When present, IgG antibody to glomerular basement membrane (GBM) antigen detected by either indirect fluorescent antibody (IFA) or multiplex bead assay helps support a diagnosis of Goodpasture syndrome. However, the combined result of both assays performed during initial evaluation improves the diagnostic sensitivity for disease. A positive result in one or both assays should be confirmed by renal biopsy.

Compliance Statement D: For laboratory tests using a manufactured RUO kit. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Note
Hotline History
N/A
CPT Codes

86255

Components
Component Test Code* Component Chart Name LOINC
0049191 GBM Antibody, IgG by IFA 29994-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Anti-GBM Antibody
  • Anti-Glomerular Basement Membrane Antibody
  • GBM IgG IFA
Glomerular Basement Membrane Antibody, IgG (IFA)