Ordering Recommendation

Preferred panel for detecting glomerular basement membrane (GBM) antibodies in suspected or established anti-GBM disease. If positive, may be useful for monitoring treatment response.


Semi-Quantitative Multiplex Bead Assay/Qualitative Indirect Fluorescent Antibody




1-5 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Storage/Transport Temperature


Unacceptable Conditions

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval
Test Number
Reference Interval
0051000 Glomerular Basement Membrane Antibody, IgG by Multiplex Bead Assay
19 AU/mL or less Negative
20-25 AU/mL Equivocal
26 AU/mL or greater Positive

0049191 Glomerular Basement Membrane Antibody, IgG (IFA) Negative

Interpretive Data

When present, IgG antibody to glomerular basement membrane (GBM) antigen detected by either indirect fluorescent antibody (IFA) or multiplex bead assay helps support a diagnosis of Goodpasture syndrome.  However, the combined result of both assays performed during initial evaluation improves the diagnostic sensitivity for disease. A positive result in one or both assays should be confirmed by renal biopsy.

Compliance Statement D: For laboratory tests using a manufactured RUO kit. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Hotline History
CPT Codes

83516; 86255

Component Test Code* Component Chart Name LOINC
0049191 GBM Antibody, IgG by IFA 29994-1
0051000 GBM Ab, IgG by Multiplex Bead Assay 44446-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Anti-GBM Antibody-IgG
  • Antiglomerular Basement Membrane Antibody-IgG
  • Basement Membrane Antibody
  • Basement Membrane, Glomerular Antibody
  • GBM Antibody
  • Glomerular Anti-Basement Membrane
Glomerular Basement Membrane Antibody, IgG by Multiplex Bead Assay and IFA