Ordering Recommendation

Preferred panel for detecting glomerular basement membrane (GBM) antibodies in suspected or established anti-GBM disease. If positive, may be useful for monitoring treatment response.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Storage/Transport Temperature


Unacceptable Conditions

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)


Semi-Quantitative Multiplex Bead Assay/Qualitative Indirect Fluorescent Antibody




1-5 days

Reference Interval

Test Number
Reference Interval
  GBM Ab, IgG by Multiplex Bead Assay 0-19 AU/mL
  GBM Antibody, IgG by IFA Negative

Interpretive Data

When present, IgG antibody to glomerular basement membrane (GBM) antigen detected by either indirect fluorescent antibody (IFA) or multiplex bead assay helps support a diagnosis of Goodpasture syndrome.  However, the combined result of both assays performed during initial evaluation improves the diagnostic sensitivity for disease. A positive result in one or both assays should be confirmed by renal biopsy.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Component Interpretation
Glomerular Basement Membrane Antibody, IgG by Multiplex Bead Assay 19 AU/mL or less
20-25 AU/mL
26 AU/mL or greater

Compliance Category

Laboratory Developed Test (LDT)


Hotline History


CPT Codes

83516; 86255


Component Test Code* Component Chart Name LOINC
0049191 GBM Antibody, IgG by IFA 29994-1
0051000 GBM Ab, IgG by Multiplex Bead Assay 44446-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Anti-GBM Antibody-IgG
  • Antiglomerular Basement Membrane Antibody-IgG
  • Basement Membrane Antibody
  • Basement Membrane, Glomerular Antibody
  • GBM Antibody
  • Glomerular Anti-Basement Membrane
Glomerular Basement Membrane Antibody, IgG by Multiplex Bead Assay and IFA