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Platelet Antigen Genotyping Panel
3000193
Ordering Recommendation

Assess risk for fetal and neonatal alloimmune thrombocytopenia. May be ordered for parental, fetal, or neonatal genotyping.

Mnemonic
HPA GENO
Methodology
Polymerase Chain Reaction/Fluorescence Monitoring
Performed
Varies
Reported
2-7 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Fetal Genotyping: Amniotic fluid OR cultured amniocytes. If the client is unable to culture amniocytes, this can be arranged by contacting ARUP Client Services at (800) 522-2787. 
WITH Maternal Cell Contamination Specimen
(see Note): Lavender (EDTA), Pink (K2EDTA), or Yellow (ACD Solution A or B).
Parental Genotyping:
Lavender (EDTA). 
Specimen Preparation
Amniotic Fluid: Transport 10 mL unspun fluid. (Min: 5 mL)
Cultured Amniocytes:
Transport two T-25 flasks at 80 percent confluency filled with culture media. Backup cultures must be retained at the client's institution until testing is complete.
Maternal Cell Contamination Specimen:
Transport 3 mL whole blood. (Min: 1 mL)
Whole Blood (Parental Genotyping):
Transport 3 mL whole blood. (Min: 1 mL) 
Storage/Transport Temperature
Amniotic Fluid: Room temperature.
Cultured Amniocytes:
 CRITICAL ROOM TEMPERATURE. Must be received within 48 hours of shipment due to liability of cells.
Whole Blood or Maternal Cell Contamination Specimen:
Refrigerated. 
Unacceptable Conditions
 
Remarks
 
Stability
Fetal Specimen: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Whole Blood or Maternal Cell Contamination Specimen:
Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable 
Reference Interval
Interpretive Data
Background Information for Platelet Antigen Genotyping Panel:
Characteristics:
Spontaneous fetal intracranial bleeding may occur in 20 percent of pregnancies affected with severe perinatal alloimmune thrombocytopenia (PAT); there is a risk of fetal death. Post-transfusion purpura may occur in transfusion recipients with antibodies to a specific platelet antigen.
Incidence:
PAT occurs in 1 in 5000 births.
Inheritance:
For women homozygous for the less common "b" HPA allele with antibodies to the common "a" allele, there is a 50 percent risk a pregnancy will be affected if her partner is heterozygous for the "a" allele and 100 percent risk if her partner is homozygous for the "a" allele.
Cause:
Maternal-fetal HPA incompatibility.
Polymorphisms Tested:
HPA-1 (ITGB3, GPIIIa) c.176T>C, p.L59P; HPA-2 (GP1BA, GPIba) c.482C>T, p.T161M; HPA-3 (ITGA2B, GPIIb) c.2621T>G, p.I874S; HPA-4 (ITGB3, GPIIIa) c.506G>A, p.R169Q; HPA-5 (ITGA2, GPIa) c.1600G>A, p.E534K; HPA-6 (ITGB3, GPIIIa) c.1544G>A, p.R515Q; HPA-15 (CD109, CD109) c.2108C>A, p.S703Y
Clinical Sensitivity:
Variable, dependent on ethnicity.
Methodology:
PCR followed by fluorescent monitoring.
Analytic Sensitivity and Specificity:
99 percent.
Limitations:
Bloody amniotic fluid specimens may give false-negative results because of maternal cell contamination. Diagnostic errors can occur due to rare sequence variations.
Informed consent:
Recommended; forms are available at www.aruplab.com.

PA 1-6, 15 Polymorphism
HPA System"a" Allele Common"b" Allele Variant
HPA 1TC
HPA 2CT
HPA 3TG
HPA 4GA
HPA 5GA
HPA 6GA
HPA 15CA

Compliance Statement C: For human genetic inheritable conditions and mutations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Counseling and informed consent are recommended for genetic testing. Consent forms are available online.

Note
Maternal specimen is recommended for proper test interpretation if contamination of the fetal specimen from the mother is suspected. Order Maternal Cell Contamination.
Hotline History
View Hotline History
CPT Code(s)
81105; 81106; 81107; 81108; 81109; 81110; 81112
Components
Component Test Code*Component Chart NameLOINC
3001171Platelet Antigen Geno Interpretation
3001173Platelet Antigen 1 Genotyping
3001174Platelet Antigen 2 Genotyping
3001175Platelet Antigen 3 Genotyping
3001176Platelet Antigen 4 Genotyping
3001177Platelet Antigen 5 Genotyping
3001178Platelet Antigen 6 Genotyping
3001179Platelet Antigen 15 Genotyping
3001180Platelet Antigen Geno Specimen
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • HPA platelet antigen genotyping panel