Buprenorphine and Metabolites, Urine, Quantitative
Ordering Recommendation

Preferred test to follow-up presumptive results. For general screening, Buprenorphine, Urine Screen with Reflex to Quantitation (2012273) is preferred.

Quantitative Liquid Chromatography-Tandem Mass Spectrometry
1-4 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Random urine. 
Specimen Preparation
Transfer 2 mL urine with no additives or preservatives to an ARUP Standard Transport Tube. (Min: 1 mL) 
Storage/Transport Temperature
Room temperature. 
Unacceptable Conditions
Ambient: 1 week; Refrigerated: 1 month; Frozen: 3 years (Avoid repeated freeze/thaw cycles) 
Reference Interval
Effective August 17, 2015
Drugs CoveredCutoff Concentrations
Buprenorphine2 ng/mL
Norbuprenorphine2 ng/mL
Buprenorphine glucuronide5 ng/mL
Norbuprenorphine glucuronide5 ng/mL
Naloxone100 ng/mL

Interpretive Data
Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry

Positive cutoff:
Buprenorphine 2 ng/mL
Norbuprenorphine 2 ng/mL
Buprenorphine glucuronide 5 ng/mL
Norbuprenorphine glucuronide 5 ng/mL
Naloxone 100 ng/mL

For medical purposes only; not valid for forensic use.

The presence of metabolite(s) without parent drug is common and may indicate use of parent drug during the prior week. Naloxone is included to detect the addition of a naloxone-containing drug directly into the urine.

The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, diluted/adulterated urine, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Hotline History
CPT Code(s)
Component Test Code*Component Chart NameLOINC
2002750Norbuprenorphine, Urn, Quant49753-7
2002752Buprenorphine, Urn, Quant3415-7
2005863Naloxone, Urn, Quant77207-9
2010093Buprenorphine Gluc, Urn, Quant
2010094Norbuprenorphine Gluc, Urn, Quant49751-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Buprenex (Buprenorphine & Metabolites - Confirmation/Quantitation - Urine)
  • Buprenorphine (Buprenorphine & Metabolites - Confirmation/Quantitation - Urine)
  • Buprenorphine Glucuronide (Buprenorphine & Metabolites - Confirmation/Quantitation - Urine)
  • Buprenorphine Screen & Metabolite (Buprenorphine & Metabolites - Confirmation/Quantitation - Urine)
  • Butrans (Buprenorphine & Metabolites - Confirmation/Quantitation - Urine)
  • Norbuprenorphine (Buprenorphine & Metabolites - Confirmation/Quantitation - Urine)
  • Norbuprenorphine Glucuronide (Buprenorphine & Metabolites - Confirmation/Quantitation - Urine)
  • Norspan (Buprenorphine & Metabolites - Confirmation/Quantitation - Urine)
  • Pain Management (Buprenorphine & Metabolites - Confirmation/Quantitation - Urine)
  • Pain Management, Buprenorphine, Quantitative, w/ medMATCH, Urine (Buprenorphine & Metabolites-Confir
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  • Suboxone (Buprenorphine & Metabolites - Confirmation/Quantitation - Urine)
  • Subutex (Buprenorphine & Metabolites - Confirmation/Quantitation - Urine)
  • Temgesic (Buprenorphine & Metabolites - Confirmation/Quantitation - Urine)
  • Transtec (Buprenorphine & Metabolites - Confirmation/Quantitation - Urine)
  • Vetergesic (Buprenorphine & Metabolites - Confirmation/Quantitation - Urine)