Bordetella pertussis Antibodies, IgA, IgG, and IgM by Immunoblot
Ordering Recommendation
If serology is used to assess late-stage pertussis, the recommended test is Bordetella pertussis Antibodies, IgA and IgG by ELISA with Reflex to Immunoblot (2001774). CDC first-line tests for pertussis are PCR (0065080) and/or culture (0060117).
Qualitative Immunoblot
1-8 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
Specimen Required
Patient Preparation
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL)  
Storage/Transport Temperature
Unacceptable Conditions
Heat-inactivated specimens.  
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year  
Reference Interval
Bordetella pertussis Antibodies, IgA, IgG, and IgM by Immunoblot
Effective February 19, 2013
 Components Reference Interval
Bordetella pertussis Antibody, IgG by Immunoblot   
 Bordetella pertussis Ab, IgG by Immunoblot Interp Negative
 B. pertussis, IgG Immunoblot PT100 Negative
 B. pertussis, IgG Immunoblot PT Negative
 B. pertussis, IgG Immunoblot FHA Negative
Bordetella pertussis Antibody, IgA by Immunoblot   
 Bordetella pertussis Ab, IgA by Immunoblot Interp Negative
 B. pertussis, IgA Immunoblot PT Negative
 B. pertussis, IgA Immunoblot FHA Negative
Bordetella pertussis Antibody, IgM by Immunoblot   
 Bordetella pertussis Ab, IgM by Immunoblot Interp Negative
 B. pertussis, IgM Immunoblot PT Negative
 B. pertussis, IgM Immunoblot FHA Negative
Interpretive Data
This assay tests for the presence of pertussis toxin (PT), pertussis toxin PT 100 (PT-100), and filamentous hemagglutinin antibody (FHA).
CPT Code(s)
86615 x3
Component Test Code*Component Chart Name
2004317B. pertussis Ab, IgA Immunoblot Interp
2004318B. pertussis, IgA Immunoblot PT
2004319B. pertussis Ab, IgG Immunoblot Interp
2004320B. pertussis, IgG Immunoblot FHA
2004321B. pertussis, IgG Immunoblot PT
2004322B. pertussis, IgG Immunoblot PT100
2004323B. pertussis Ab, IgM Immunoblot Interp
2004324B. pertussis, IgM Immunoblot FHA
2004325B. pertussis, IgM Immunoblot PT
2004397B. pertussis, IgA Immunoblot FHA
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • B. pertussis Abs immunoblot
  • Pertussis Antibody
  • Pertussis IgA,IgG, IgM Abs Immunoblot