Bordetella pertussis Antibodies, IgG and IgM by ELISA with Reflex to Immunoblot
2001784
 
Ordering Recommendation
If serology is used to assess late-stage pertussis, the recommended test is Bordetella pertussis Antibodies, IgA and IgG by ELISA with Reflex to Immunoblot (2001774). CDC first-line tests for pertussis are PCR (0065080) and/or culture (0060117).
Mnemonic
BORDPAN
Methodology
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
Performed
Tue
Reported
1-8 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory.
Specimen Required
Patient Preparation
 
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Contaminated, heat-inactivated, or severely lipemic specimens.  
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year  
Reference Interval
Test Number Components Reference Interval
2001768Bordetella pertussis Antibody, IgG by ELISA with Reflex to Immunoblot
Test Number Components Reference Interval
Bordetella pertussis Antibody IgG by ELISA Effective August 19, 2013
0.0-​0.9 U/mL
2004327Bordetella pertussis Antibody, IgG by Immunoblot Refer to report
2001769Bordetella pertussis Antibody, IgM by ELISA with Reflex to Immunoblot
Test Number Components Reference Interval
Bordetella pertussis Antibody, IgM by ELISA 0.9 U/mL or less: Negative -​ No significant level of detectable Bordetella pertussis IgM antibody.
1.0-​1.1 U/mL: Equivocal -​ Repeat testing in 10-​14 days may be helpful.
1.2 U/mL or greater: Positive -​ IgM antibody to Bordetella pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis.
2004326Bordetella pertussis Antibody, IgM by Immunoblot Refer to report
Interpretive Data
Recommend that treatment decisions be based on the result of the B. pertussis IgM immunoblot test instead of the ELISA test. B. pertussis IgM test by ELISA may produce false-positive results.
Note
If Bordetella pertussis Antibody, IgG by ELISA is 1.0 U/mL or greater, then Bordetella pertussis IgG Immunoblot testing will be added; if Bordetella pertussis Antibody, IgM by ELISA is 1.2 U/mL or greater, then Bordetella pertussis IgM Immunoblot testing will be added. Additional charges apply.
CPT Code(s)
86615 x2; if reflexed, add 86615 for each Immunoblot
Components
Component Test Code*Component Chart Name
2001782B. pertussis Ab, IgG by ELISA
2001783B. pertussis Ab, IgM by ELISA
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • Pertussis Antibody
  • Pertussis IgG, IgM Reflex to immunoblot