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Bordetella pertussis Antibodies, IgG and IgM by ELISA with Reflex to Immunoblot
2001784
Ordering Recommendation

If serology is used to assess late-stage pertussis, the recommended test is Bordetella pertussis Antibodies, IgA and  IgG by ELISA with Reflex to Immunoblot (2001774). CDC-recommended first-line tests for pertussis are Bordetella pertussis/parapertussis by PCR (0065080) and/or Bordetella pertussis Culture (0060117).

Mnemonic
BORDPAN
Methodology
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
Performed
Tue, Fri
Reported
1-5 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Serum separator tube. 
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent." 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Contaminated, heat-inactivated, or severely lipemic specimens. 
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year 
Reference Interval
Test Number
Components
Reference Interval
2001768Bordetella pertussis Antibody, IgG by ELISA with Reflex to Immunoblot
Test Number
Components
Reference Interval
Bordetella pertussis Antibody IgG by ELISAEffective February 20, 2018
0.94 IV or less: Negative - No significant level of detectable B. pertussis IgG antibody.
0.95-1.04 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.05 IV or greater: Positive - IgG antibody to B. pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis.
2004327Bordetella pertussis Antibody, IgG by ImmunoblotRefer to report

2001769Bordetella pertussis Antibody, IgM by ELISA with Reflex to Immunoblot
Test Number
Components
Reference Interval
Bordetella pertussis Antibody, IgM by ELISAEffective February 20, 2018
0.9 IV or less: Negative - No significant level of detectable B. pertussis IgM antibody.
1.0-1.1 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.2 IV or greater: Positive - IgM antibody to B. pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis.
2004326Bordetella pertussis Antibody, IgM by ImmunoblotRefer to report


Interpretive Data
Recommend that treatment decisions be based on the result of the B. pertussis IgM immunoblot test instead of the ELISA test. B. pertussis IgM test by ELISA may produce false-positive results.

Compliance Statement A: For laboratory developed tests using a manufacturer labeled ASR as the reagent providing the specificity of the assay. Analyte Specific Reagent. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.

Note
If Bordetella pertussis Antibody, IgG by ELISA is 1.05 IV or greater, then Bordetella pertussis IgG Immunoblot testing will be added; if Bordetella pertussis Antibody, IgM by ELISA is 1.2 IV or greater, then Bordetella pertussis IgM Immunoblot testing will be added. Additional charges apply.
Hotline History
View Hotline History
CPT Code(s)
86615 x2; if reflexed, add 86615 for each Immunoblot
Components
Component Test Code*Component Chart NameLOINC
2001782B. pertussis Ab, IgG by ELISA42330-1
2001783B. pertussis Ab, IgM by ELISA42329-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Pertussis Antibody
  • Pertussis IgG, IgM Reflex to immunoblot