Do not use to determine thiamine deficiency.
Quantitative High Performance Liquid Chromatography
This test is New York DOH approved.
- Patient Preparation
- Green (sodium or lithium heparin), lavender (EDTA), or pink (K2 EDTA).
- Specimen Preparation
- Separate plasma from cells within one hour of collection. Protect from light within one hour of collection, and during storage and transport. Transfer 3 mL plasma to an ARUP Amber Transport Tube. (Min: 0.2 mL)
- Storage/Transport Temperature
- Frozen. Separate specimens must be submitted when multiple tests are ordered.
- Unacceptable Conditions
- Hemolyzed specimens or specimens other than heparin or EDTA plasma.
- After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen at -20°C: 6 months
Total thiamine, measured as thiamine (vitamin B1) and thiamine monophosphate, is reported. However, thiamine diphosphate (TDP), the biologically active form of the vitamin, is best measured in whole blood and is not found in measurable concentration in plasma. Plasma thiamine concentration reflects recent intake rather than body stores.
See Compliance Statement B: www.aruplab.com/CS
Whole blood is the preferred specimen for thiamine assessment. Approximately 80 percent of thiamine present in whole blood is found in red blood cells. Refer to Vitamin B1 (Thiamine), Whole Blood (0080388).
|Component Test Code*||Component Chart Name|
|0080389||Vitamin B1, Plasma|
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
- B1 Vitamin
- Thiamine monophosphate
- Vitamin B1 (Thiamine),